Browsing by Author "Tadesse G/giyorgisG/giyorgis, Tadesse G/giyorgis"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Stripping Square Wave Voltammetry for the Determination of Uric Acid in Human Urine Using Fe3+ Doped Zeolite Graphite Powder Composite Modified Glassy Carbon Electrode
    (Addis Ababa Universty, 2011-06) Tadesse G/giyorgisG/giyorgis, Tadesse G/giyorgis; Tessema, Merid (PhD)
    A novel stripping square wave voltammetric method has been developed for the determination of uric acid (UA) in untreated human urine using iron (III) doped zeolite/graphite powder composite modified GCE. The synergetic effects of the catalytic role of Fe3+, larger surface area of zeolite, and higher conductivity of graphite powder account for the anodic peak current obtained at much lower positive oxidation potential than at the bare GCE. The proposed method is simple, rapid, has excellent selectivity, reproducible and sensitive in the linear range of 2–80 μM UA with a detection limit of 2.3 x 10-7 mol L-1. The developed method enabled to achieve recoveries ranging from 90.98 to 106.77% of spiked standard UA from untreated human urine with a standard error of less than unity. Key words: Zeolite NaY (zeolite), Iron doped zeolite, undoped zeolite, stripping SWV, chemically modified electrode (CME), uric acid (UA), human urine, polystyrene, dichloroethane, tetrahydrofuran (THF)