Browsing by Author "TAMRAT, TOMAS"

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    COMPARISON OF CLINICAL TRIALS OF BOVINE MASTITIS WITH THE USE OF ANTIBIOTICS AND HONEY
    (2007-06) TAMRAT, TOMAS; Prof. A.R.S. Moorthy
    A total of 423 representative randomly selected Holstein, Jersey and local zebu lactating cows located in Wolaita zone at Soddo Zuria Woreda of Southern Nation Nationalities and Peoples Regional State were examined. All the 46 lactating cows of Soddo dairy farm, 11 lactating cows from Soddo Veterinary Clinic, 103 lactating cows from urban dairy holders and the rest 263 were X randomly selected lactating dairy cows from 18 rural Kebeles were included and tested to determine the prevalence of mastitis by using mastitis detecting BOVI-VET (RUUSE, DENMARK), indicator paper and white side test. Milk samples were cultured from 329 cows infected with different grades of mastitis. Examination for mastitis infection in Soddo Zuria Woreda revealed that there were 99 (23.4 %) clinical and 230 (54.3 %) sub clinical cases. Out of 423 cows 99 clinical cases and 230 sub-clinical cases were recorded and grouped into grade I, II, III, IV level of infection and only clinical cases were subjected to antibiotics and honey intramammary infusion treatment. The prime objective of the study is to examine the safety and efficacy of honey compared to a known antibiotic infusion in lactating mastitic cows. Minimum Inhibitory Concentration (MIC) of honey was determined by using different dilutions and different species of bacteria available at the microbiology laboratory of FVM. And it was revealed that minimum concentration of honey that inhibited E. coli was 10 %; L. monocytogenes and K. pneumoniae inhibited at 20 %; Staphylococcus and Streptococcus spp, were inhibited at 30% concentration, where as Micrococcus spp. at 40 % concentration. Salmonella enteritidis was resistant (shown growth in all concentrations). Out of ninety-nine clinical cases, forty-eight cases were treated with antibiotic (Multiject IMM.) infusion and fifty cases were treated with Beza honey. Milk samples from the cows identified and recorded for treatment trial groups were re-cultured within one month post-treatment. Cases included in the analysis had at least one mastitis pathogen isolated from the initial milk samples. Overall bacteriological cure rate of treated cases was 54 % (53 of 98). The effectiveness of honey in clearing bacterial infection in the intramammary infusion for mastitis treatment revealed similar to antibiotic (54.2% (Multiject IMM.) treated cases. Antibiotic and non-antibiotic treatment responses were associated with grade of affection. Honey treatment was more effective 78.1% in grade III type of mastitis when compared to treatment of grade II type of mastitis (11.1%); where as multiject was more effective in grade II type of mastitis (41.9%) and slightly less curative rate (76.4%) for grade III type of mastitis when compared to honey. The present study indicated that honey treatment could be an alternative treatment to antibiotics so that indiscriminate use of antibiotics and emergence of antibiotic resistance strains of microorganisms could be avoided and at the same time financial benefits can be obtained. Treatment with honey is more economical i.e.7-8 times cheaper than the available mastitis treatment drugs in the country. As an alternative to antibiotic treatment for XI mastitis, 10 ml of 40% honey intramammary infusion for each quarter for 3 consecutive days can be tried.