Browsing by Author "Mussa Daniel"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Suitcase Laboratory for Rapid Detection of SARS-CoV-2 Virus Based on Recombinase Aided Isothermal Amplification Assay.
    (Addis Ababa University, 2024-06-19) Mussa Daniel; Desta Kassu
    Introduction: Techniques such as RT-PCR are not only expensive and time-consuming but also require a high level of expertise, limiting their widespread deployment, particularly in resource constrained regions. Mobile Suitcase Laboratory equipped with Recombinase Aided Isothermal Amplification assay, is an alternative approach which potentially overcomes those challanges by providing a rapid, cost-effective, and user-friendly assay for the diagnosis of COVID-19. Therefore this study aimed to evaluate the diagnostic accuracy of a point of need Mobile suitcase laboratory based on Recombinase Aided isothermal amplification assay for diagnosis of SARS-CoV-2 virus. Methods: A combination of retrospective and prospectivecross-sectional study was conducted adhering to established guidelines for the assessment of diagnostic kits, aiming to evaluate the performance of a cost-effective and straightforward isothermal assay in comparison to the standard RT-PCR method for diagnosing SARS-CoV-2. From archived samples, 290 positive samples were selected based on their CT values, along with 86 negative samples for the study. Additionally, 98 positive and 52 negative fresh samples were added. In total, 388 positive samples and 138 negative samples were used. Statistical Packages for Social Sciences (SPSS) version 22.0. Results: Of the total, 388 individuals tested positive with the RT PCR, 334 (86.0 %) were also positive with tool kit. Additionally, 138 individuals tested negative with the RT PCR, 133 (96.4%) of them also negative with the Tool kit. The Kappa statistic (0.7) and the low . Conclusion: This study evaluating the Mobile Suitcase Laboratory (MSL) based on Recombinase Aided Isothermal Amplification (RAA) assay showed its potential utility in resource-limited settings such as Ethiopia. Our findings demonstrate that the MSL-RAA assay offers a promising alternative for decentralized COVID-19 testing, overcoming the infrastructure and logistical challenges associated with laboratory-based methods like RT-PCR. Key words: Rapid test, diagnosis, RT-PCR, Recombinase, SARS-Cov-2