Browsing by Author "Mohamed Israel"

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    Nematicidal Activity of the 80% Methanol Extract and Major Compounds Isolated from the Unripe Fruits of Peponium vogelii (Hook. f.) Engl.
    (Addis Ababa University, 2022-04-12) Mohamed Israel; Asres Kaleab
    Helminthiasis remain a major public health problem affecting an estimated 1.45 billion individuals worldwide, particularly in tropical developing countries. The aim of this study was to investigate the nematicidal activity of 80% methanol extract of unripe fruits of Peponium vogelii Hook. f. Engli. (Cucurbitaceae), a medicinal plant used to treat intestinal parasite infection in Southwest Ethiopia, and determine the compounds responsible for activity using bioassay-guided fractionation. 80% methanol extract of unripe fruit of P. vogelii was first fractionated by using solvents of different polarity followed by a silica gel column chromatography and reverse phase HPLC, while nematicidal assay was followed throughout on Caenorhabditis (C.) elegans as a model organism. Results of the study revealed that the 80% methanol extract of the unripe fruits of P. vogelii was active against C. elegans (p < 0.001) with an IC50 value of 23.5 μg/ml. Bioassay-guided fractionation led to the isolation of five active components designated PVMF2-4, PVMF2-5, PVMF2-7, PVMF2-8, and PVMF2-9, which exhibited percentage inhibition of 84%, 96%, 97%, 89% and 41%, respectively. Based on spectroscopic data (1H and 13C NMR and ESI-MS), PVMF2-8 and PVMF2-8 which were active and major components from active fraction were identified as linoleic acid and palmitic acid respectively. The results support the use of P. vogelii unripe fruits for the treatment of intestinal parasite infection by traditional healers in Southwest Ethiopia.