Browsing by Author "Metages, Yirgalem"

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    MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUSES IN CATTLE FROM OUTBREAKS OCCURRED IN DIFFERENT PARTS OF ETHIOPIA FROM OCTOBER, 2017 TO MAY, 2018
    (2018-06) Metages, Yirgalem; Dr. Fufa Dawo, Dr. Bedaso Mamo; Dr. Daniel Gizaw
    A cross sectional study with purposive sampling was conducted with the aim of molecular characterization of foot and mouth disease virus in cattle from outbreaks occurred in different parts of Ethiopia from October, 2017 to May, 2018. Outbreaks were investigated and individual animals were clinically examined. Samples were collected for virus isolation, serotyping and molecular characterization. A total of 125 animals from different outbreak sites were examined clinically for the presence of the disease. Of which, 56 (44.8%) animals were manifested clinical signs and lesions suggestive of FMD. From 37 clinical samples collected during the study period, only two serotypes (O and A) were identified using antigen detection ELISA and in 15/22 (68.18%) samples virus were isolated on BHK-21 cell. FMDV genome was also detected in 12/ 27 (44.44%) samples by real time RT-PCR. A total of 18 representative samples from both serotypes were submitted to world reference laboratory for FMD (WRLFMD), Pirbright, UK for confirmation and molecular characterization based on viral protein 1 (VP1) sequencing. The result of phylogenetic analysis revealed that, the current isolates of serotype O belonged to East Africa topotype -3 (EA-3) and the serotype A was clustered in African topotype of genotype -I (G-I). In addition, serotype O isolate shared 96.6% nucleotide similarity with Sudan’s isolates while serotype A shared >97% nucleotide similarity with Kenyan and Tanzanian isolates which are grouped previously under African topotype of genotype I which indicates the presence of unrestricted animal movement. G-I is reported for the first time in Ethiopia. A total of 10.8% amino acid variations were recorded when VP1 sequence of the vaccine strain (O/ETH/38/2005) was compared with serotype O isolate in this study. Most of the variations were observed at amino acid position 133-158 and 194-213, which is known to be the immunodominant region. Thus, to enhance control of FMD in Ethiopia, detailed molecular analysis of outbreaks coupled with in-vitro vaccine matching to determine protecting potential of the vaccine strain currently in use is recommended. Key words: Ethiopia, FMD, Serotype A and O FMD viruses, Molecular characterization, Outbreak investigation.