Browsing by Author "Meskerem Adamu"

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    Study on Comparative Diagnostic Techniques of Fasciolosis in Slaughed Ruminants at Elfora Export Abattoir Debre Zeit ,Ethiopia
    (Addis Ababa University Faculty Of Veterinary Medicine, 2006-06) Meskerem Adamu; Getachcw Tilahun
    A stud y was conducted at EL FO RA Export Aballoir. Dcbrc·Zeit in order to compa re the d iffe ren t di agnos ti c tec hniq ues in nl1 11 in anl fa sc iolo sis. T he results of standa rd faeca l sedim entat ion techni que fo r nuke egg quant ifi cat ion was compa red to th a t of an l!llzymc linked immu nosorbcll t assay (indirect ELI SA ) les t and liver inspect io n as we ll as res u lts of SenllTI enzyme activ ities. Out o f th e 324 call1 e , 350 sheep and 385 goats s laughtered at ELFORA Export Abatto ir , 125 (38.5%), 4 ( l.l-Jo~o) and 2 (0.5 10'0) were found \0 be posni\-e for lesions of fasc io losis, re specti ve ly duri ng post mortem in specti on. Dur ing faeca l exam ination 108(33%) , 4( 1. 14%) and 2(0. 5 1%) wc re pos iti ve for fascio la eggs in catt lc ,sheep and goats respec tively. Furt her examinatio n on 13.f ran d om ly selected samples from eanlc revea led that 75%, 29°0 and 4 1% we re positi\'e lIs ing sero logical, fa ecal and postmortem examination techn iques. respec ti vel y. In sma ll nuninant s . the preva le nce of fasclo losls \\IIS low botb in coproscopy a nd indirec t ELI SA testes: on ly one o ut of 68 sheep was positive coproscopicall y a nd J we re posi tiv e sero logi cally: and one a liI of 22 goats tested was po::,itl\ e both at coproscopy and se rology exam ina ti ons. Ti ll S indicates the high scmlllvity of ELISA compared to the others techniques in d iagnos ing fascio los is. The o \'era ll assessment indicatl!s the relatedness of faeca l examina ti on result w ith ELISA find ings. though thc test agreement I::, weak ( Kappa 0.236). SimJiariy. there was a strong relationship between faecnl e'\ammatlon and postmortem findings o f liver lesions, Kappa value . P=0.758). Out of the 5-l canle \\Hh known li\'er lesions of fasc iola 33 (6 1. 1%) were ha\lng e levated Ic\cls of GGT than l10nmil \'aluc for caule. m support of the above statement, our findmgs regardmg the kvcl of GGT suggest its association wi th li ver lesions encounte red at postmortem examination. The k\ cl of test of agrccment between the liver exa mination and GGT determination \\as howc\ er moderate (Kappa=0.332). On the other hand the level of LOll has shown to bc gcnerall) ele\ atcd both in negative as we ll as pos itive anllnals ror live r lesions mdicatmg the absence of relationship between liver les ions and level of LDI L The use 01 LliSA IS found to be a more reliable d iagnostic lest than coproscopy in detecting fasciolosis III ruminants
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    Study On Comparative Diagnostic Techniques of Fasciolosis in Slaughtered Ruminants at Elfora Export Abattoir Debre Zeit, Ethiopia
    (2006-06) Meskerem Adamu; Getachew Tilahun
    A study was conducted at ELFORA Export Abattoir, Debre-Zeit in order to compare the different diagnostic techniques in ruminant fasciolosis. The results of standard faecal sedimentation technique for fluke egg quantification was compared to that of an enzyme linked immunosorbent assay (indirect ELISA) test and liver inspection as well as results of serum enzyme activities. Out of the 324 cattle, 350 sheep and 385 goats slaughtered at ELFORA Export Abattoir, 125 (38.5%), 4 (1.14%) and 2 (0.51%) were found to be positive for lesions of fasciolosis, respectively during post mortem inspection. During faecal examination 108(33%), 4(1.14%) and 2(0.51%) were positive for fasciola eggs in cattle sheep and goats respectively. Further examination on 134 randomly selected samples from cattle revealed that 75%, 29% and 41% were positive using serological, faecal and postmortem examination techniques, respectively. In small ruminants, the prevalence of fasciolosis was low both in coproscopy and indirect ELISA testes: only one out of 68 sheep was positive coproscopically and 3 were positive serologically; and one out of 22 goats tested was positive both at coproscopy and serology examinations. This indicates the high sensitivity of ELISA compared to the others techniques in diagnosing fasciolosis. The overall assessment indicates the relatedness of faecal examination result with ELISA findings, though the test agreement is weak (Kappa 0.236). Similarly, there was a strong relationship between faecal examination and postmortem findings of liver lesions, Kappa value, P=0.758). Out of the 54 cattle with known liver lesions of fasciola 33 (61.1%) were having elevated levels of GGT than normal value for cattle, in support of the above statement, our findings regarding the level of GGT suggest its association with liver lesions encountered at postmortem examination. The level of test of agreement between the liver examination and GGT determination was however moderate (Kappa=0.332). On the other hand the level of LDH has shown to be generally elevated both in negative as well as positive animals for liver lesions indicating the absence of relationship between liver lesions and level of LDH. The use of ELISA is found to be a more reliable diagnostic test than coproscopy in detecting fasciolosis in ruminants.