Browsing by Author "Mekonnen Wondwossen"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    In vitro and In vivo Immune Responses in Ethiopian Tuberculosis Patients with HIV Infection
    (Addis Ababa University, 2001-06) Mekonnen Wondwossen; Petros Beyene (PhD); Wolday Dawit (PhD)
    Mycobacteriulll tuberculosis and HIV - I arc known to interact with each other with an impact on the immunological, virological/bacterial and clinical outcomes of both infections. We aimed to evaluate the ill vitro and ill vivo immunolgical alterations in TB and HlV-1 co-infection. The study population consisted of 101 subjects: HIV- and HlV+ individuals with TB (HlV-TB+, n=29; HIV+TB+, n=31), individuals with HIV only (HlV+TB-, n=19), and healthy controls (HlV-TB-, n=22). Lymphoproliferative responses were evaluated in mitogen- or antigen-stimulated PBMCs by BrdU ELISA. Cytokines (IFN-y, IL-lO, and IL-12) in cell culturc supell1atants of PBMCs, and plasma concentrations of TNF-a and TNF-RII werc measured by sandwich ELISA. Plasma viral load was detelmined by nucleic acid sequence-based amplification; and C04+ T-cells were enumerated by FACScan. Results showed that C04+ cell counts (cells/mm3) were significantly reduced in individuals with TB and/or HlV infections compared to the healthy controls. Patients co-infected with TB/HIV had a significantly elevated plasma viral load (loglO copies/ml) as compared to HIV+ without TB. Proliferative responses were reduced in TB+HlV- (1.36 and 0.58 to PHA and PPO, respectively), TB-HlV+ (1.17 and 0.40) as well as those with TB+HlV+ (1.13 and 0.47) compared to healthy controls (1.76 and 0.83). IFN-y production was significantly depressed in TB+HIV- (P