Browsing by Author "Hadgu Endale"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Molecular Characterization and Assessment of Viral Tumorigenesis in Breast Cancer among Women in Addis Ababa, Ethiopia
    (Addis Ababa Universty, 2017-07) Hadgu Endale; Seifu Daniel
    In this study from Tikur Anbessa Specialized Hospital, 114 breast cancer patients diagnosed between 2012 and 2015 were enrolled. Estroge receptor (ER), Progesterone Receptor (PR), Ki 67 and Human Epidermal Growth factor (HER-2) receptor status were assessed using immunohistochemistry (IHC) from tissue microarrays (TMA). Fluorescence in situ hybridization (FISH) and Gene Protein Assay (GPA) was used for assessment of gene amplification in all equivocal tumor samples and for confirmation in HER2-enriched cases. Androgen Receptor (AR) was assessed using IHC from TMA and BRCA1 was assessed using IHC from whole section. EBV, HCMV and HPV viral proteins or/and DNA were assessed using IHC or/and multiplex qPCR. The 2013 St. Gallen international panel of expert’s recommendation for classification of breast carcinoma based on IHC was applied to molecularly classify the tumors. Information obtained also included age, tumor grade, histological type, and stage of disease. In this study, the most common molecular subtypes was Luminal A (40%) followed by Luminal B (26%), TNBC (23%) and HER2-enriched (10%). ER were positive in 65% of all tumors and 43% the participants were positive for PR. There was statistically significant variation in median age at diagnosis between the different molecular subtypes There was a bimodal distribution of molecular subtypes in different age ranges at diagnosis with Luminal B subtype being more common at younger ages (median=36) and Luminal A subtype being more prevalent at older ages (median=42). There were no statistically significant differences in tumor grade, histology, and stage between the molecular subtypes of breast cancer. AR was expressed in 80% of breast cancers, which is higher than the expression rates of both ER and PR. There was a statistically significant variation Key words: Breast cancer, Molecular subtypes, AR, BRCA1, Ki67, oncogenic viruses, Ethiopia, Africa
  • No Thumbnail Available
    Item
    Molecular Characterization and Assessment of Viral Tumorigenesis in Breast Cancer among Women in Addis Ababa, Ethiopia
    (Addis Ababa University, 2017-07) Hadgu Endale; Seifu Daniel
    In this study from Tikur Anbessa Specialized Hospital, 114 breast cancer patients diagnosed between 2012 and 2015 were enrolled. Estroge receptor (ER), Progesterone Receptor (PR), Ki 67 and Human Epidermal Growth factor (HER-2) receptor status were assessed using immunohistochemistry (IHC) from tissue microarrays (TMA). Fluorescence in situ hybridization (FISH) and Gene Protein Assay (GPA) was used for assessment of gene amplification in all equivocal tumor samples and for confirmation in HER2-enriched cases. Androgen Receptor (AR) was assessed using IHC from TMA and BRCA1 was assessed using IHC from whole section. EBV, HCMV and HPV viral proteins or/and DNA were assessed using IHC or/and multiplex qPCR. The 2013 St. Gallen international panel of expert’s recommendation for classification of breast carcinoma based on IHC was applied to molecularly classify the tumors. Information obtained also included age, tumor grade, histological type, and stage of disease. In this study, the most common molecular subtypes was Luminal A (40%) followed by Luminal B (26%), TNBC (23%) and HER2-enriched (10%). ER were positive in 65% of all tumors and 43% the participants were positive for PR. There was statistically significant variation in median age at diagnosis between the different molecular subtypes There were loss/decreased BRCA-1 protein expression in 29% of all cases. No association was found between altered nuclear BRCA1 expression and ER expression. BRCA-1 expression was not associated with AR, HER2 and Ki67. There was no statistically significant difference in BRCA-1 expression among the molecular subtypes of breast cancer in this study. No association was found with age at diagnosis, tumor grade, clinical stage and histological type of tumor. Out of 113 participants 44 cases (39 %) had high Ki67 protein expression. High Ki67 was found to be significantly associated with median age Key words: Breast cancer, Molecular subtypes, AR, BRCA1, Ki67, oncogenic viruses, Ethiopia, Africa