Browsing by Author "Guta Korsa"

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    Electrical Properties of two Hours Treated 4T1 Breast Cancer Cells and Effect of Magnetic Beads on Ionization of BT20 Breast Cancer Cells Using Optical Twezeers
    (Addis Ababa University, 2024-09) Guta Korsa; Endris Mohammed
    Study was undertaken to introduce the novel optical tweezers method for exploring the electrical charge developed and trap coefficient of pre-ionized 2 hour treated 4T1 breast cancer cells and to assess how magnetic beads affect the ionization properties of BT20 breast cancer cells. Specifically, the research focused on 4T1 breast cancer cells, which had been treated with the chemotherapy drug DMDD extracted from Averrhoa carambola L., and BT20 breast cancer cells mixed with 3.1 μm diameter polymer-coated magnetic beads. The trapping was achieved using an infrared diode-pumped Nd:YVO4 laser with a wavelength of 1064 nm, maximum output power of 8W, beam diameter of 4 mm, and a highly focused, linearly polarized beam. In experiments with the pre-ionized 2 hour treated 4T1 breast cancer cells, we recorded the following average values; the charge generated on cells in Z-numbers of 432.60±688.77, angular frequency of 72.12±30.12 rad ⁄s and trap coefficient of 8.32±4.55 un/m. As the cells approached to the center of the trap, their motion was influenced by the opposing of the drag force and the electrostatic force generated by the induced electric field, that resulted in their deceleration. When magnetic beads inserted in BT20 breast cancer cells they affected their ionization characteristics as the cells stayed in the trap.