Browsing by Author "Fentahun, Tarekegn"

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    Production of Gluconic Acid from Cane Molasses Through Submerged Fermentation Using Aspergillus Carneus
    (AAU, 2018-06) Fentahun, Tarekegn; Anuradha, Jabasingh (PhD)
    Gluconic acid is a multifunctional organic acid which has increasing interest in different application because of its non-corrosiveness, non-toxicity, readily biodegradability and environmentally friendly properties. This study involves gluconic acid production and investigation of different parameters on the yield of gluconic acid. Gluconic acid was produced from treated molasses by using Aspergillus carneus strain under submerged fermentation. To reduce the inhibition of heavy metals and inorganic salts in crude molasses, it requires being pretreated. Gluconic acid production was achieved by three subsequent steps such as pretreatment, fermentation and separation with characterization. To investigate the effect of variables, four factors with three levels Box -Bhenken Design expert 7.0.0 trial was used. Chemical characterization of gluconic acid was examined by Fourier transform infrared spectroscopy analysis. Results of Box -Behnken Design indicates that the optimum parameter were temperature 30 , Carbon source concentration 250.00g/L, pH 7 and incubation time 6.86 days to produce maximum yield of gluconic acid 61.96%. Fourier transform infrared spectroscopy analysis identifies and confirms the presence of gluconic acid in the product. Results showed that use of treated molasses for production of maximum yield of gluconic acid through submerged fermentation by using Aspergillus carneus were valuable, feasible and waste minimal.