Browsing by Author "Berhe, Asefaw"

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    Molecular Epidemiology of HIV -1 Infection in a Cohort of Ethiopian Factory Workers
    (Addis Ababa University, 1999-06) Berhe, Asefaw; Rinke, Tobias (PhD)
    In this study, to investigate the molecular epidemiology of the HIV-I virus in Ethiopia, the HIV -I gp 120 V3 loop and flanking nucleotides were amplified from viruses isolated frolll the plasma samples of23 I-IIV positive participants in the ongoing EHNRI-ENARP cohort project in Akaki and Wonji factories. A nested PCR DNA amplification protocol yielded correct size products, as determined by agarose gel electrophoresis in 61 out of 75 (81 %)plasma samples collected between 1996-1999. Out of these 61 samples, 53 (87%) were sequenced successfully and analysed. Phylogenetic analysis determined that all V3 sequences are of the HIV-1 subtype C, the most predominant in Ethiopia. Also, the V3 sequences derived showed lower intra-person mutation frequencies than inter-person mutation fi•equencies. In addition, it could be demonstrated that the Wonji and Akaki epidemics have a common origin and do not originate fi'om separate introductions. Finally, the proportions of non-synonymous (dn) versus synonymous (ds) mutations are variable per subject and are not dependent on CD4 counts or viral load.