Browsing by Author "Ashenafi, Hagos"

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    Serological and Epasitologlcal Survey of Dourilne (Trypallosoma Equiperdum) in Selelected Sites of Ethiopia
    (Addis Ababa University Faculty of Veterinary Medicine, 2000-06) Ashenafi, Hagos; Abebe, Professr Getachew
    The present stud} wa~ conducted rrom August 2004 to Apnl 2005 \\ L1h Iht: ObJccll\e~ IIf dctemumng. the prevalence, extent and d istribution 0 dourine III horses and IO\CSlIgaling occurrence of cllmeal cases 3S well 3S carner st:ltes of donkey .. and mules III the Arst- B:llc Illgh la nd s. At the same time a lon g Ltudmal study was conducted to assess the efficacy of the Isomet::mudlUlll chlonde ( IS MM ) Hnd D lITu nazenc acetumte (I)I~ I ) III Iht treatment of ch mcal c ases of dou rine. An IIlvcs ligalion was also conducted encom pa~Slllg cluesttonmure nnd serological survey o n th e presence of dO Urine in adjacent geograph ic"l areas of the Ar<;I- Ha1c highlands and other sclec ted parts o f the country with lugh equlOe population, The stud) methodol ogy was based on quest io nn:lIrc, serological. climeal and pa rasitologica l surve~, The questionnaire survey indi cated th ai do urine, locally known, as " Lappessa" or " D irr C$s a·· is a major health problem of equ ines in th e Arsi-Bale hi g hlands Though, dourine 1<; a common clinical case th rough Qut th e year, it has a seasonal character. \\ hieh most com monly occur" following Ih t' b reeding !;l' aSOIl from Junc to late Septembe r. SOIllC!ltne~ a <;('coml peak IS observcd III Ihe dl) seasons of the ~ car (Marc h to t>. o r any other haemopara~l1c~ could be detected in all cxanll ned Glemsa stained smears (th in blood. gell ital di sc harge and ti~l;uC fluids) as well as In bl ood samples by mil CT. All th e puppIes Inoc ulated \\lth blood samrk~. gellll.tl wa she s and oed cmalOUS nUids remained parasl1ologicall ) Ilegau\e. Diffe rent ch ara ctcri stic clinical signs o f dourinc were observed in clilllcally ~Ic k horses of both sexes . The prominent clinical signs observed as geni tal fo m) o f the di sease mclude vagmal dl:.chargc mallll~ of mucopurulent type "ilh foul odou r. oedema lind presence of deplgmcn ted ..,cars 0\ (' 1' till' o,:)'lerna l genita lia , oc,icmu o f Ihe <;cro ltlm and prepuce aecomp:llllcd by pn:pucl.:t1 ali \\ell .h urethral d Isch arge and ukl'rallon of the gen ita l m ucosae mlllnl~ nf the- pen tie In hoth "'C\l' ~ . lamencs~ in one or both leg ... panml dr;tgg ln g and slIffnc"s of thl' lund Icg~ and Itlcoordtnilth)n \H·r~· lhc dominant sig n .. nbscrv o.!d , I ~ nervous fo rm of lhe dl ~CIl<;C viii Horses with different ehOlc al signs of doun nl! were categonsed Into one of the followi ng fi\e grou[ls based on th e cl llll cal findin gs Apparc llIl y health y (46 81. non -s pccliic s ign s like ema ciat ion ilnd weakne ss (69); ge nital fonn (30); nervou, fonn 01 [15) and bo th gCTll1ul .lnd nervous forms of th e disease (67). Fifty - four horses with hi gh pCrtenl :l ge [lO !.I U\,II)' fOl the md lrec t anllbody ELI SA and mn eleen horses wllh stron g agglullnall un reactlOTlS having end III fe of 1.32 for both CA l-r and LATEX tes ts be longed to th e liw of th ... dlffeT1:nl dUlleal group~ ChI-sq uare analysIs of the di s tributi on of anImals. claSSIfied liS C) tl Wf POS III\ e llr negative 011 Ihe ba sis ofallthi." three teSt s. in th e clull eal grou ps demon st rat ed a s tat l<;tltally slgm fi cn nt d iffe ren ce between gro ups. with s tratum specific prevalence o f po siuvc amma i<; mc rca!'llllg wit h In crc aslIlg severity of clmieal sign .. (p of I SM~1 and Dl:V1 III selected serologically po slI1\·e cli nical cases of Cou nnl.' 111 A<; as<;a dl !'llTh:l of th e Ar !') hlghl.lIlds A lot.11 of 24 adull 10e:11 horses were divided Inl O ~ group, of cq11al number I' CV k\ cl. eos!l1opllll cou nt , body temperature, se rologi cal sWt u". unrf\)\emcni of the C'I'tlllg clinical !'Ilgn~ Jnd bod) w ndition ~Cl)rc \\ en: moni tored tWIce m 10 d):" 1I1Ienal fClr a penod til 40 dll) ~ rhe res ults IIldu:ated th at mean ]l eV, eosinophil counts and t>"dy Icmperaturl' impw\ ed in bo th J S\ I~I and D I ~I treated group during th e 40 day s pO St tr(';ll meni penod A ~ Ia nsth:,ill ~ sig nifi can t Increase in PCV and body temperature was observed ~1.lrIlIIg frOlll th e day 10 ( P kilO .... n ende mi C fOCI of the ArSI-Ualc hIghlands of I:thIOPI:l ,\llhough thcre wa~ no d,rect detection of T C'qU/f)('rdllfll, there IS 'mong eVidence Ihal dounne IS highly pre\:llclll, mo,t Important problem LIl th e Arsi- llaic hIghlands. whe re the Sllua tlon IS gellmg .... orse \\ Llh the preo;cOI spreading trend to areas previously known as free of the disease. neces,"alC~ urgent nall onal control intrevtion
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    Studies on Bovine Trypanosomosis and Efficacy of Selected Trypanocidal Drugs in Konso District, Southern Ethiopia
    (Addis Ababa University, 2008-06) Ayledo, Gewado; Ashenafi, Hagos
    Studies on bovine trypanosomosis and efficacy of selected trypanocidal drugs involving field and experimental investigations were undertaken inK onso district, southern Ethiopia, from September 2007 to April 2008, with views to determine the prevalence a nd socioeconomic impacts of bovine trypanosomosis; assess the efficacies of selected trypanocidal drugs; and evaluate the propensity of Konso community to devote public resources to integrated tsetse/trypanosomosis control approaches. Questionnaire interviews, cross-sectional and experimental study designs were applied to collect relevant information. A structured questionnaire was designed and posed to randomly selected households and key informants to identify areas with high suspicion of drug resistance. Questions addressed main issues like: herd structure and major livestock health problems; socioeconomic impacts of trypanosomosis; the sources, usage pattern and suspected failure of trypanocidal drugs, etc. Open-ended and close-ended questionnaire interviews were administered to randomly selected households to evaluate the propensity of the community to a holistic integrated disease control. In order to identify areas with high trypanosome infection pressure and risk of drug resistance, initial prevalence study was conducted in representative sample of cattle by examination of monthly blood samples through micro- haematocrit centrifugation and Buffy coat methods. The relationship between parasitological prevalence of trypanosomal infections and herd mean pev was im·estigated through haematological examination during the rainy and dry seasons. In order to assess the therapeutic and prophylactic efficacies of the common trypanocidal drugs, ten zebu calves (Bos indicus) were experimentally infected with randomly selected field isolates of T. congolensc and, when parasitaemic, treated with Diminazene aceturate and Isometamidium chloride at dose rates of 3.5 and 0.5 mglkg body weight. respectively. Experimental animals were monitored for clinical and parasitological parameters on regular basis for over three months. The study results revealed trypanosomosis to be a major threat to livestock production with contrasting arrays of socioeconomic impacts; significant reductions in cattle production losses after tsetse control and a corresponding rise in mean holdings of draft oxen and use of animal traction over the same period: an indiscriminate use and increasing tendencies in mean annual expenditure on trypanocidal drugs at the household level. Contingent valuation study disclosed animus propensity of VI integrated tsetse/trypanosomosis control; household SIze, wealth status and educational background of household heads to be the major determinants influencing willingness to support disease control. Cross-sectional study suggested an overall prevalence of 17.8 % and 14.2 % during rainy and dry season, respectively, reflecting its significant temporal and spatial variation (p< 0.001); and T congolense to be a dominant trypanosome species hampering livestock subsector in Konso district. Regression analyses on haematological findings disclosed a significant reduction (p<0.05) in the herd mean rcv with an increase in the prevalence of trypanosomosis; and that the reduction in herd rcv was significantly higher during dry season than in rainy season (p<0.001), suggesting that trypanosomosis is less-well tolerated during dry months. Results of drug sensitivity testil1g revealed the presence of T congolense populations exhibiting resistance to Diminazene aceturate and, possibly to Isometamidium chloride. In conclusion, the absence of improved veterinary service and indiscriminate use of poor-quality trypanocidal drugs have proven to boost the risk of drug resistance in Konso district. In light of the high likelihood of trypanocidal drug resistance in Ethiopia, the present findings could be a useful tool to improv~ trypanocidal drug usage strategies in the field, and could form baseline information to undertake holistic assessments of drug resistance across tsetse-infested areas of Ethiopia. It is recommendec that integrated disease control approaches be adopted with chemotherapy restricted to clinicall: sick animals, and legislations be devised and harmonized to ensure the quality of trypanocid2 drugs. Keywords: Community participation; Drug resistance; Integrated approach: Sensitivity te~ Southern Ethiopia; Livestock; T congolense; Trypanocidal drugs.