Browsing by Author "Agedew, Tewodros"

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    Evaluation of the anticonvulsant activity of 80% methanol leaf extract and solvent fractions of Buddleja polystachya Fresen. (Buddlejacea) in Mice
    (Addis Ababa Universty, 2018-03) Agedew, Tewodros; Nedi, Teshome (PhD)
    Evaluation of the anticonvulsant activity of 80% methanol leaf extract and solvent fractions of Buddleja polystachya Fresen. (Buddlejacea) in mice. Tewodors Agedew Addis Ababa University, 2018 Buddleja polystachya is used in Ethiopian traditional medicine for the treatment of epilepsy. Since this claim has not been investigated scientifically, this study was conducted to evaluate the anticonvulsant activity of 80% methanol leaf extract and solvent fractions of Buddleja polystachya in mice. The leaves of plant were extracted using maceration technique; butanol and chloroform were used for fraction. Anticonvulsant activity was evaluated by using maximal electroshock (MES) and Pentylenetetrazol (PTZ) model. Motor coordination effect was assessed by using rotarod test. Mice were randomly assigned to five groups (n=6 per group). The test groups received 100 mg/kg, 200 mg/kg and 400 mg/kg of cure extract and solvent fraction. The positive control groups received phenytoin 25mg/kg for MES test, valproate 200mg/kg for PTZ test and diazepam 5mg/kg for rotarod test. The negative control groups received distilled water or 2% Tween80, 10 ml/kg. The crude extract exhibited significant anticonvulsant effect in both MES test (p<0.001 for all doses) and PTZ test (p<0.001 for 200 mg/kg and 400 mg/kg, p<0.05 for 100mg/kg) compared with control. A similar effect was observed with butanol fraction in both models. Whereas, the chloroform fraction showed significant (p<0.001) anticonvulsant effect relative to control only in PTZ test at doses of 200mg/kg and 400mg/kg. The aqueous fraction was devoid of anticonvulsant activity in both seizure models. No significant changes in motor coordination were detected in all doses of the crude extract and solvent fractions. The plant extract contained flavonoids, phenols, tannins, steroids, terpenoids and saponins. This study indicated that the plant has a promising anticonvulsant activity, and it could be considered as a potential source to develop new anti-epileptic drug.