Browsing by Author "Agari Feyisa"

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    Isolation and Antimicrobial Susceptibility Profile of Listeria Monocytogenes in Municipal Abattoir and Butcher Shops in Addis Ababa.
    (Addis Abeba University, 2023) Agari Feyisa; Bedaso Mammo (PhD); Zerihun Beyene(DVM, MSc)
    Listeriosis, which is caused by Listeria monocytogenes, is one of the most common foods borne zoonotic infection having significant public health concerns. The persistence of L. m onocytogenes in the food production environments and consumption of contaminated food of animal origin such as beef has great public health significance in Ethiopia where raw meat has always been considered as traditional delicacy. Therefore, a cross-sectional study was conducted from December 2022 to May, 2023 to isolate L. monocytogenes from raw b eef meat and environmental samples at Addis Ababa abattoirs enterprise and butcher shops in Addis Ababa and assess its antimicrobial susceptibility pattern. A total of 280 samples consisting of carcass and environmental samples were collected from abattoir (n=150) and butcher shops (n=130) in the study area for microbiological analysis of this bacteria. After primary and secondary enrichments with demi Fraser and Fraser broth, all collected sampl es were subjected to 3M molecular detection assay for detection and then presumptive posi tive was transferred to Biolog identification system (GEN III MicroPlate) for confirmation according to manufacturer’s protocol. The overall prevalence of L. monocytogenes was found to be 4.64% (13/280) of which 4.67% (7/150) were from abattoirs and 4.62% (6/130) were from butchers’ shops. Out of 120 beef carcass samples collected 5.83% (7/120) were positive for L. monocytogenes. In this study the occurrence of L. monocytogenes was found to be 6% (3/50), 3.64% (2/55) and 4% (1/25) from personnel hands, knifes and cutting boards respectively. All L. monocytogenes isolates were subjected to antimicrobial susceptibility testing using eight available antibiotic disks. Out of 13 isolates, 92.3% (12/13) were equally resistant to tetracycline and amoxicillin whereas 84.6 % (11/13) and 76.9% (10/13) were resistant to streptomycin and ampicillin respectively. However, all isolates were found to be susceptible to Trimethoprim-sulpha methazole. Eleven (84.6%) isolates were found to be multi-drug resistant. This study showed that there was occurrence of antibiotics resistance developed against L. monocytogenes in Addis Ababa beef value chains, which requires improved good manufacturing and hygienic practices during meat production, HACCP im plementation, prudent use of antimicrobial drugs and regular surveillance system.