Hundhammer, B.(PhD)Berhanu, Tarekegn2018-06-252023-11-092018-06-252023-11-091994-06http://etd.aau.edu.et/handle/12345678/3141AC and DC voltammetry and flow injection amperometry methods were employed in this work. The influence of ionophores on the facilitated transfer of K+ and Na+ ions across membrane stabilized water/nitrobenzene interface was investigated. According to this investigation benzo 15 crown 5 (B15C5) was found best for K+ ion and dibenzo 24 crown 8 (DB24C8) for Na+. B15C5 was an appropriate ionophore in simultaneous determination of K+ and Na+ ions. DB24C8 was found to be poor in this regard. Stability constants of metal-ionophore complex were estimated. The values forK+ -ionophore (K-DB24C8, KDB18C6) were in good agreement with literature values, while those of Na+-ionophores (Na-DB24C8, Na-DB18C6) are higher than the literature values. Concentrations of Na+ and K+ in blood serum were determined simultaneously using flow injection amperometry and flame photometry. The results obtained from voltammetric and flow injection amperometric investigations indicate that the membrane stabilized interface is a promising as a voltammetric sensor in clinical analysis of blood electrolytesenAC and DC VoltammetryThesis