Ayalew,Yohanne(RN, PhD Fellow) ,Adimasu,Mekonen (BSc, N, MSc, N,)Alemayehu,Feven2023-12-092023-12-092023http://etd.aau.edu.et/handle/123456789/498Background: The most common cause of morbidity and mortality in neonatal intensive care units around the world is nosocomial or healthcare associated infections. Up to 40% of reported neonatal mortality in developing countries is attributed to nosocomial infections, which have a 30% incidence in neonatal critical care units. Objective: The aim of this study was to assess knowledge, practice and associated factors towards prevention of hospital acquired infection among nurses working in neonatal intensive care unit of selected governmental hospitals Addis Ababa, Ethiopia. Method: Hospital based cross-sectional study was conducted from March 21 – April 21, 2022. Census method was used to obtain the study participants and self-administered questionnaires were used to collect data from 155 participants in selected five governmental hospitals in Addis Ababa, Ethiopia. Data was entered using EPI info version 7.0 statistical software and analyzed using SPSS version 26 statistical package. Bivariate and multivariable logistic regression analysis was also carried out to identify factors associated with the dependent variable. A p-value less than 0.05 were used to declare statistical significance. Result: Around half (53.5%) of nurses were found to have good knowledge and 56.9% of respondents demonstrated a good practice on infection prevention. Reading guide lines, receiving formal training, working experience, marital status, availability of personal protective equipment and hand hygiene equipment, and availability of infection prevention guide lines were significantly associated (P < 0.05) with knowledge and practice of nurses towards prevention of hospital acquired infection. Conclusion and Recommendation: About more than half of nurses had good knowledge and good practice on hospital acquired infection prevention. This implies significant proportion of nurses had poor knowledge and practice towards prevention of infection. Therefore, institutions should provide trainings and improving sustainable supplies like personal protective equipment and hand washing equipment, and encouraging nurses to read and use infection prevention guideline.en-USThesis