Wordofa, BerhanuAlemu,TsionMamuye, Ephrem2024-01-012024-01-012023http://etd.aau.edu.et/handle/123456789/1336Background; Cervical cancer is caused by HPV and it is the primary cause of illness and mortality in females around the globe. It ranks the second most common cancer-related cause of death for women in Ethiopia. Although ministry of Health recommends two doses of HPV vaccine, the uptake status of HPV-2 is very low as compared to HPV-1 that hinder of the full protection from HPV Infection. Objectives: To assess the uptake of second dose HPV vaccine and factors associated with it among adolescent girls in Worabe town, Southern Ethiopia, 2023. Methods: Institution-based mixed study design was conducted from February 20 to March 20, 2023. A total of 406 female students were recruited by computer generated simple random sampling for quantitative study and 8 female students were recruited by purposive sampling method for qualitative study and data saturation was used as a guide to decide number of participants. The data was entered into Epi Data version 4.6. and analyzed by the use of SPSS version 26 for quantitative data and manual thematic analysis was done for qualitative data. To find associations, both bivariate and multivariate logistic regression analysis were used. Result: At all a lower level 128(31.5%) of adolescent girls had received second dose HPV vaccine. Primary educational level of adolescents[AOR =3.70, 95%CI:(1.58-8.68)], fathers who had no formal education[AOR=0.12,95%CI:(0.03-0.52)], fathers attended primary educational level [AOR=0.30,95%CI:(0.14-0.67)], not aware towards HPV-2[AOR=0.46,95%CI:(0.24-0.89)], poor knowledge of HPV and its vaccine[AOR=0.57,95%CI:(0.33-0.99)] and negative attitude towards HPV vaccine[AOR =0.37,95%CI:(0.21-0.65)] were the factors associated with the uptake of HPV-2 in the quantitative study. In addition to this poor perception of severity, lack of pre- information, lack of awareness of the gap between HPV-1 and HPV-2, absence of health worker for the second vaccine, fear of injection, fear of side effects, misunderstanding about HPV vaccine and poor awareness of HPV-2 were the explored factors in the qualitative study. Conclusion and Recommendation; Generally, there was low uptake of second dose HPV vaccine in the study area. Therefore, in order to increase second dose HPV vaccination uptake level, awareness raising and behavior modification education are essential in the community.en-USHPV, HPV vaccine, HPV-2 Uptake, Worabe townThesis