Prof.Makonnen, Eyasu (PhD)Dr. Umer, Shemsu (PhD)Dr.Arayaselassie, Mahlet (MD)Duga, Gudeta2021-05-232023-11-062021-05-232023-11-062021-02http://etd.aau.edu.et/handle/123456789/26528Liver diseases are the major worldwide health problem about 50% of which is drug-induced hepatotoxicity. Cordia africana Lam. (Boraginaceae) is widely used in Ethiopian folk medicine for treatment of different types of liver disorders. The aim of this study was to investigate hepatoprotective effects of aqueous (CAAE) and 80% methanol extracts of C. africana stem bark (CAME) and the solvent fractions of the CAME against acetaminophen (APAP)-induced liver injury in rats. APAP, 2 g/kg given orally, was used to induce liver injury. Blood samples were taken and serum levels of liver enzymes, and total bilirubin (TB) as well as lipid profiles were determined. Histopathological examination of liver tissues was conducted to confirm the findings of biochemical analysis. Intra-peritoneal (I.P) sodium pentobarbital (SPB)-induced sleep duration was also used to determine the protective effect of the test substances. Oral administration of APAP (2 g/kg) resulted in a significant increase in serum levels of alanine aminotransferase (ALT) (227.80±40.80), aspartate aminotransferase (AST) (417.73±67.10), alkaline phosphatase (ALP) (248.33±34.55), TB (0.19±0.05), low-density lipoprotein (LDL) (13.03±1.70), total cholesterol (TC) (45.95±3.30) and triglycerides (TGs) (114.35±12.14) and decrease in serum high-density lipoprotein (HDL) (19.90±3.44). The levels of all these biomarkers were reversed by the standard drug, silymarin 100 mg/kg. Administration of both crude extracts at different doses and fractions also reversed serum levels of all the parameters tested to ward normal. CAME exerted significant dose-dependent hepatoprotective effects in terms of ALT and AST, while the CAAE showed similar activity in terms of AST, ALP and TGs. The results of histopathological investigations confirmed the hepatoprotective effects shown in the biochemical analysis. C. africana stem bark crude extracts and the solvent fractions of the methanol extract showed hepatoprotective effects.en-USCordia africana, Ethiopia, Blood biomarkers, Liver enzymes, Lipid profiles, Liver disease, Hepatoprotective effect, Acetaminophen, Silymarin.Thesis