Chandravanshi B.S. (Professor)Tassew Zebasil2018-06-272023-11-092018-06-272023-11-092009-03http://etd.aau.edu.et/handle/12345678/4160In this paper the level of nicotine in four different varieties of Ethiopian tobacco leaves was determined using High Performance Liquid Chromatography (HPLC) method of analysis. Among the variety of species Virginia tobacco leaves were found to have high nicotine level (3.26 %), followed by the native tobacco ‘Gaya’ (1.1 %) then burley tobacco leaves (0.65 %), finally oriental tobacco leaves were found to have lowest nicotine level, though its actual level of nicotine was not determined .Moreover it was confirmed that the nicotine level of Ethiopian tobacco leaves increases from bottom to top leaf (stalk) positions of the tobacco plant, as well it was found that nicotine level of the same species tobacco differ based on area of cultivation. The levels of nicotine in Ethiopian tobacco (obtained by this study) were found to be comparable with that in the rest of the worldenEthiopian Tobacco LeavesThesis