Seleshi ZelekeSelam Aysheshim2026-07-022026-07-022025-05https://etd.aau.edu.et/handle/123456789/8592This study examined the relationship between knowledge, attitudes, and motivations with donation practices among regular and lapsed donors in Addis Ababa. A mixed-method study was conducted at the Ethiopian Red Cross Society Blood Donation Center. Quantitative data were collected through structured questionnaires from 200 voluntary donors (66% regular, 34% lapsed), while qualitative interviews supplemented the findings. Most participants were males (69%), aged 26–35 years, and held undergraduate degrees (66%). About 74% demonstrated adequate knowledge, and all respondents held favorable attitudes toward blood donation. Major motivators for first-time donation included influence from friends and relatives (37.5%) and donation campaigns (17%). Factor analysis identified four motivational domains: altruistic values, personal rewards, self-esteem, and social influence. A chi-square and independent t-test showed that regular donors have significantly higher knowledge, attitude, and service perception scores than lapsed donors. Logistic regression showed that attitude (OR = 1.246, p < .001) and value-based motivation (OR = 2.292, p < .001) significantly predicted regular donor status. Knowledge had a marginal association (p = .069), while reward, esteem, and social motives were not significant predictors. Value based appeals, better service experiences, and improving donor education could improve donor retention strategies in Ethiopia. Keywords: knowledge, attitude, blood donation, VFIenKeywords: knowledgeattitudeblood donationVFIThesis