Gebre-Selassie SolomonLemma Ayalew2022-06-072023-11-052022-06-072023-11-052012-06http://etd.aau.edu.et/handle/123456789/31943Back ground: Infectious diseases are diseases that are transmitted mainly through foodand still remain a common and persistentpublic healthproblem resulting high morbidityand occasional mortality in both developed and developing countries. Foods obtainedfrom animals play an important role for food borne diseases particularly in developing countries like Ethiopia. Food borne diseases arecaused by a variety of bacteria, viruses, parasites and fungi. Salmonellosis is one of those food borne diseases caused by Salmonella species which is becoming resistant to antimicrobials and causing serious diseases especially in developing countries because of consumption of raw foods such as meat, milk and cheese. Objective: This study is aimed to determine the prevalence of Salmonella species from foods of cattle source and perform their anti-microbial susceptibility tests. Materials and Methods: A cross-sectional study was conducted over a period of 4monthsfrom November, 2011 to February, 2012 in Merkato, Addis Ababa Ethiopia. Inthis study, a total of 384 samples from meat, milk and cheese were collected randomly from the Market and transferred to the laboratory following the ISO-6597, 2002procedure. In order to isolate Salmonella species, conventional culture method such aspre-enrichment, enrichment and selective plating were performed. Then, to confirm theidentification ofisolated coloniesasSalmonella Species, TSI, motility and API 20E tests were used. Finally, anti-microbial susceptibility testing following the standard procedure was performed in order to know the pattern of drug resistant for each isolate and the results were analyzed and interpreted by using SPSS 16.0,2007 computer Software. Results: Out of 384 food samples consisting of 128 meat, 128 milkand 128 cheesesamples, 13 (3.39%) were positive forSalmonella spp.Of the 128 meat, 128 milkand128 cheese samples, 9 (7.03%), 3(2.34%) and 1 (0.78%) yielded Salmonella spp.respectively.Assay of antimicrobial resistance revealed that 100% of Salmonellaisolateswere resistant to one or more of the 10 antimicrobials tested. Generally,resistance for 9different antimicrobial drugs was recognized. However, all of the isolates were sensitiveto ciprofloxacin. The most common resistance was observed to amoxicillin(100%)followed by ampicillinand tetracycline (76.9%) and most of Salmonella spp.isolates aremulti-drug resistance(84.6%). Conclusions and Recommendations:The findings of the present study ascertain thatSalmonellaspp. is isolated in food samples ofcattle sources particularly in retail meatsamples and all of them have developed resistance for routinely prescribed antimicrobialdrugs and this may be a considerable risk to the consumers. So,prudent antimicrobialusage, adequate heat treatment, improvement of standards of hygiene and developmentand enforcement of suitable legislation which safeguard consumers are urgentlyinstituted and must be practiced to combat the ever increasing situation of anti microbial resistance.en-USSalmonella,antimicrobial resistance, food items, Merkato,Addis Ababa,Ethiopia.Thesis