Tsegaye, Aster ( PhD)Alemu, Rahel(BSc, MSc)Mamushet, Yared(MD)Kebede, Henok ( MSc )Tamire, Tewodros( MSc)Aschenik, Mulualem2021-12-122023-11-062021-12-122023-11-062021-08http://etd.aau.edu.et/handle/123456789/29209Background: Analysis of body fluids provides important information for assessing various medical conditions. Bothe manual and automated hematology analyzers are used for cell counting. However, no published study is available to validate the diagnostic performance of the Sysmex 4000-i (Sysmex, Japan) system for the analysis of CSF and Pleural fluids. Objective: To compare the manual counts of cerebrospinal fluids (CSF) and pleural fluids (PF) with the Sysmex XT-4000i automated hematology analyzers. Methods: In this cross-sectional study, 240 body fluid samples were analyzed using the Sysmex 4000-i system in the body fluid mode and light microscopy at Tikur Anbessa Specialized Hospital. Body fluids included were pleural and cerebrospinal fluid (CSF). The results of the manual and automated counts were compared using the Pearson correlation test. Results: A comparison between the Sysmex 4000-i system and manual counting demonstrated great correlations with regard to pleural fluid WBC, Polymorph nuclear cells and Mono nuclear cell counts where r =0.82, 0.96, 0.95, respectively in both methods of the counts. Cell count from CSF also showed good correlations for both manual and automated counts of WBC, PMN % and MN% where r=0.83, 0.94, and 0.92, respectively. The Sysmex 4000-i system also demonstrated good performance for differential cell counting (r =0.9028). CSF particularly showed a good correlation. Conclusions: The use of the Sysmex 4000-i system for cell counting and differential analysis of body fluid samples might be an effective and automated alternative to chamber counting in laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.en-USCSF, Pleural fluid, Manual count, automated count.Thesis