Abate Dawit (PhD)Tsegaw Dawit2018-06-262023-11-082018-06-262023-11-082009-08http://etd.aau.edu.et/handle/123456789/3591In a screening of fungi isolates associated with litter decomposition (litotrophic) for bioactive metabolite production, LDMFS JP4 was isolated. It was identified to genus level using morphological (cultural) and microscopic characterization to belong to Geotrichum sp. A bioactive metabolite effective against bacteria and fungi, was isolated from this fungus at stationary phase of mycelium culture growing in solid substrate, rice. Through, bioassay guided fractionation of the crude extract using chromatographic methods, the active compound was isolated in pureform. The compound was found to exhibit activity. It was effective against gram positive (S. pnumoneae, S. aureus and B. subtilis) and gram negative bacteria (P. aeruginosa, E. coli and S. boydii) and the yeast, Candidia albicans. The active compound exhibited potent in-vitro antibacterial with a minimal inhibitory concentration (MIC) of 65-75μg/uL against Gram-positive bacteria and 75-80ug/uL against Gram-negative. It was less effective for the yeast, Candidia albicans. The pure compound is promising and further work on chemical characterization is recommended. KEY WORD, LDFs, taxonomic studies, solid substrate, bioactive metabolite, fractionation, minimal inhibitory concentrationentaxonomic studiessolid substratebioactive metabolitefractionationminimal inhibitory concentrationThesis