Dr.Mequanente, SolomonPro.Makonnen, EyasuDr.Adefries, BeleteMengie, Teklie2020-06-102023-11-062020-06-102023-11-062020-01http://etd.aau.edu.et/handle/123456789/21505The leaf of Achyranthes aspera L.(Telenj), has been used traditionally for the treatment of wound in var- ious parts of Ethiopia. The leaf had many confirmed in-vitro and in-vivo activities that can promotewound healing and anti-inflammatory effects. However, the various fractions of A. aspera have not been explored scientifically for its wound healing and anti-inflammatory activities in animal model. The objective of this study was to evaluate the wound healing and anti-inflammatory activities of solvent fractions of 80% methanol leaf extract of A. aspera in rats. Air dried leaf of A. aspera were grounded andmacerated for 72 hours successively by 80 % methanol three times. Then the dried extract was fractionated with chloroform, n-butanol and distilled water. Acute oral and dermal toxicity was determined in rats. Wound healing activity of solvent fractions was evaluated using excision and incision wound models. Anti-inflammatory activity was evaluated using carrageenan induced hind paw edema and cottonpellet induced granuloma models in rats. 2000 mg/kg oral and 10 % w/w solvent fraction ointment testdoses were safe in rats. The 10 % w/w chloroform fraction ointment revealed high percentage of wound contraction (p<0.01) and reduced period of epithelialization (p<0.01). In the carrageenan induced paw edema and the cotton pellet induced granuloma models chloroform, n-butanol and aqueous fractionsshowed a statistically significant effect with different onset and magnitude. Chloroform fraction was found to be the most active fraction, and the 400 mg/kg dose demonstrated the maximum percent of inhibition of edema (52.50 %; p < 0.01) at 4 hr of post induction which is comparable to indomethacin at the dose employed. Chloroform fraction was also found to be the most active fraction in inhibiting the exudative and proliferative component of chronic inflammation in the cotton pellet induced granuloma model, where the maximum percentage of exudate inhibition (37.52 %, p < 0.01) and granuloma formation inhibition (52.81 %, p < 0.01) was exhibited at the dose of 400 mg/kg. Data obtained from thepresent study collectively indicated that the chloroform fraction of 80 methanol leaf extract of A. asperapossessed significant wound healing and anti-inflammatory activities, upholding the folkloric use of the plant.en-USAchyranthes aspera, wound healing activity, anti-inflammatory, carrageenan induced paw edema, cotton pellet granuloma, rat.Thesis