Birhanu,Gebremariam (PhD)Nigatu,Muluken(Asst. Prof.)Tegegne,Aychew Mekuriaw2024-04-232024-04-232023https://etd.aau.edu.et/handle/123456789/2795Resistance to antimicrobials is one of our most significant worldwide challenges. In Africa, around 31% of the infections of urinary tract (UTIs) initiated by Escherichia coli (E. coli) have been observed to develop ciprofloxacin (CIP) resistance. As a result, significant efforts have been made to investigate novel and improved antibiotics. Nanoparticles (NPs) have been shown to have significant efficacy in medication delivery against multidrug-resistant (MDR) strains of the bacteria. Green synthesized metal oxide NPs using plant extract is eco-friendly, biocompatible, less toxic, cost-effective and rapid method to control such emergent. This study aimed at synthesizing iron oxide nanoparticles (IONPs) using banana peels (Musa Spp.) extract for delivery of CIP against resistant E. coli. The preparation of IONPs was done by green synthesis method. The extract of banana peels was employed as an agent of reduction and stabilization for the precursor ions of iron used in the manufacture of IONPs. Fourier-transform infrared (FTIR) spectroscopy, Dynamic light scattering (DLS), X-ray diffraction (XRD), ultraviolet-visible spectrophotometer (UV-vis), scanning electron microscopy (SEM), and differential thermal gravimetry (DTG) were used to characterize the manufactured and medication-loaded IONPs. DLS, UV-vis, and FTIR were once utilized to assess load capacity and encapsulating capability of IONPs. CIP-IONPs were evaluated for its efficient release of the loaded drug in three different simulation media using UV-vis spectrophotometer and for its in vitro antimicrobial susceptibility. The formation of hematite (α-Fe2O3) was confirmed with its FTIR characteristic peak for it at 461cm-1, 542cm-1 and 1131cm-1. The size of synthesized IONPs were found to be 10.4nm ± 1.98, 48nm ± 0.9 and 67.3nm ± 0.9 under XRD, SEM and DLS measurements respectively. CIP-IONPs had almost the maximum drug loading capacity (33.3% ± 0.67) with fast and slow drug release pattern at gastric and intestinal or blood pH respectively. The antimicrobial susceptibility study also showed a significant resistance reversal effect for ciprofloxacin resistant E. coli. Green synthesized IONPs were produced efficiently using a banana peels extract. It had a recommended characteristics and application. And it will be a good alternative in alleviating the emergent CIP resistant E. coli.en-USAntimicrobial resistanceGreen synthesisIron oxide nanoparticlesCiprofloxacinBanana peels extractE. coli.Thesis