Prof.Mekonnen, Eyasu(PhD, Professor of Pharmacology)Beyene, Takele(Associate Professor of Pharmacology)Yetayeh, Ayechew2021-10-132023-11-062021-10-132023-11-062021-07http://etd.aau.edu.et/handle/123456789/28116Trypanosomiasis is one of the neglected tropical diseases of both humans and animals caused by the protozoa Trypanosomes mainly endemic in Africa. Unavailability of vaccine, difficulty of vector controls, low therapeutic index of the available trypanocidal drugs, and development of resistance lead to the need for research focused on developing alternative safer, more effective and more accessible treatment options especialy from medicinal plants. In Ethiopia, many medicinal plants such as Cymbopogon citratus and Lepidium sativum are used for treatment of trypanosomiasis as traditional remedies. The objective of the present study was, therefore, to investigate antitrypanosomal activities of leaves of C. citratus and seeds of L. sativum in in vivo mice model. The acute toxicity result showed that the extracts were safe at the dose of 2000mg/kg, and the test doses were adjusted to 100, 200 and 400mg/kg based on the toxicity profile. The Plants extracts were administered to the respective groups of mice after the 12 th days of T. congolense inoculation where the peak parasitemia were achieved. The treatment continues consecutively up to seven days and the parameters were recorded every other day up to the 14 th day of treatment commenced. The in vivo test results revealed that both plant extracts had dose dependent antitrypanosomal activity. Both crude extracts showed a significant reduction in parasite load (P<0.05), ameliorated anaemia (increased or prevent the fall of PCV value) (P<0.05), decreased lymphocytosis and increased neutrophil counts (p<0.05) and improved body weight but significant body weight increment (P<0.05) was observed only in C. citratus treated mice compared to the negative and positive controls. Comparative results from all tested parameters showed that the best activities were observed with C. citratus treated groups of mice (73.2% reduction).en-USAntitrypanosomal activity, Cymbopogon citratus, Lepidium sativum, Trypanosoma congolenseThesis