Tsegaye AsterAlemu JemalDibaba Berhanu2020-11-152023-11-062020-11-152023-11-062020-06http://etd.aau.edu.et/handle/123456789/23273Background: -Physicians' medical decisions are based on information provided by laboratoryreports in the form of a reference interval (RI) or medical decision limit. The interval serves as ahealth-related standard with which to compare an individual test result. However, the lack ofbringing up as its own standard, local reference values has been a problem facing hematologicalpractice in our country. Objective: To establish hematological parameters reference intervals for apparently healthyadolescents (12-17) in Asella town, South East Ethiopia from January to March 2020 GC. Method: A total of 342 apparently healthy adolescents aged 12–17 years living in Asella town andfulfilling the eligibility criteria were recruited for this cross-sectional descriptive study using asystematic random sampling method. Data including socio-demographic characteristics werecollected using a structured questionnaire. The hematological analysis was performed usingSysmex KX-21 3 Diff analyzer. Data was entered and analyzed using SPSS version 21. The nonparametricMann-Whitney u test was used to compare the parameters between genders. The 97.5percentile and 2.5thpercentile as the upper and Lower Reference limit in favor of the adolescentpopulation were determined. Result: The study observed statistically significant mean differences between males and femalesin Hemoglobin (HGB), Hematocrit (HCT), Mean cell hemoglobin concentration (MCHC), MeanCell volume (MCV), absolute lymphocyte number (LYM#), Red Blood Cell (RBC), and Redblood cell distribution width by the coefficient of variation (RDWCV). Whereas the mean valuesof other Hematological parameters like WBC PLT and RWDSD have no significant differencebetween bothsex (p value>0.05). Hence the reference interval of White Blood Cells (WBC), Platelet (PLT) andRed blood cell distribution width by the standard deviation (RWDSD) in adolescents were (3.410.9x103/ul), (153.9-390x103/ul) and (36.8-47.1fl) respectively. The current established referenceintervals show higher proportions out of range values when compared to the existing referenceintervals. Conclusion: Some hematological parameter showed a significant difference in the mean valuesamong data sets for HGB, RBC, MCV and MCHC across gender (p value<0.05) in which femaleshaving lower levels than males. The newly established hematological parameter reference intervalsby this study were different from the existing reference values.en-USAdolescent, Hematological parameter, reference intervalThesis