Dr. Fanta Desissa, Mr. Takele BeyeneMunera, Ahmednur2019-12-192023-11-082019-12-192023-11-082019-06http://etd.aau.edu.et/handle/123456789/20439A cross-sectional study was conducted from November 2018 to May 2019 in Adama and Modjo. The main objectives of the study were to isolate and identify selected zoonotic pathogens from dairy cattle, to assess practical usage of drugs and to determine the in vitro antimicrobial resistance profiles of the isolates. Structured questionnaire was designed and administered to dairy farm owners and dairy food consumers to assess their consumption behavior and antibiotics usage. A total of 607 samples consisting of fresh cow milk, fecal sample, nasal swab, pooled milkers’ hand swabs, pooled floor swabs and tank milk samples were collected from 20 dairy farms. The samples were examined for the presence of selected zoonotic pathogens. Total of 396 samples were analyzed for S. aureus and 15.7% (62) were positive. Total of 396 samples were analyzed for E. coli and 7.6% (30) were positive. From 271of the total analyzed samples for Salmonella 4.8% (13) were positive. A total of 62 S. aureus, 30 E. coli and 13 Salmonella isolates were observed resistant to at least one or more antimicrobials tested. Penicillin, Methicillin and Sulphametoxazole trimethoprim were drugs to which a large proportion of isolated S. aureus were (100%to90%) highly resistant. From 30 tested E. coli show resistance to Tetracycline (83%) and Vancomycin (80%). The resistances of Salmonella were 69% to Nalidxic Acid and 54% to Vancomycin. Multiple drug resistance was detected in high (98.4%) for S. aureus, (56.7%) for E. coli and (53.9%) for Salmonella. High proportion of the current misuses of drugs and occurrence of resistance isolates to drugs in the dairy farms alerts concern for animal and public health as these drugs is used widely for treatment and prophylaxis in animals and humans.enDairy farmsE. coliMultidrug resistanceThesis