Ermias LulekalAssefa Belachew2025-08-182025-08-182024-06https://etd.aau.edu.et/handle/123456789/7022This research was carried out to record and document medicinal plants and associated indigenous plant use knowledge of the local community in Siyadebr and Wayu Woreda, of Amhara Reginal State, Ethiopia. The ethnobotanical data were collected by conducting prepared semi-structured interview items with 96 informants. In the district of these informants, 62 were males and 34 were females. Of these, 8 key informants were selected purposively. The data were also collected by using field observation, group discussion, and specimen collection. Determination of Informant Consensus Factor, Preference Ranking, Direct Matrix, and Fidelity Level were performed. A total of 87 medicinal plant species distributed in 78 genera and 40 families were documented. Out of these 87 plants were used to treat 58 species human ailments, 11 species livestock ailments and 18 species both human and livestock ailments. 45.98% of medicinal plants were harvested from the wild environments. Of the total medicinal plant species, 35 were herbs. The family Asteraceae and Fabaceae occupied the first rank with 8 species. The most frequently used plant parts were leaves 34.16%. Freshly harvested plant parts 61% preparations were mostly used for remedy preparation. The most widely used method of preparation was crushing 20.87%. The common route of administration recorded was oral 58.79% followed by dermal 26.92%. Agricultural expansion, fire wood, fence, and construction were the major threats to medicinal plants of the study area. Thus, a coordinated in-situ and ex-situ conservation measure is mandatory to save the fast-declining medicinal plants of the study area.en-USAilmentsEthnobotanyMedicinal PlantsSiyadebr and WayuTraditional HealersThesis