Abay MuluDagim Tsegaye2023-12-092023-12-092023-10-23http://etd.aau.edu.et/handle/123456789/485Traditional medicine is the culmination of generations of indigenous system of medicine practitioners' therapeutic experiences. Although over 2,000 plants have been identified in Africa and are utilized to cure a wide range of ailments, only few of these plants have had their safety thoroughly investigated. Ethiopians have historically utilized plants as medicine, although little is known about the characteristics and range of these traditional practices. Objective, The aim of this study was to Evaluate of acute and subacute dermal toxicity of aerial part Cymbopogon nardus formulation on hematological, clinical chemistry and histopathology of skin, kidneys and liver of Albino Wistar rats. Methodology, The plant material was harvested about 270 kilometers south of Addis Ababa, in the Wondogenet region surrounding Shashemene town. The Armaeur Hansen Research Institute's (AHRI) traditional and modern medicine research directorate provided the formulation. Essential oil extraction was undertaken by taking the fresh plant material and subjecting it to hydro-distillation for 2 hours. The plant used Clevenger-Arm equipment to carry out the hydro-distillation process. A round-bottom flask weighing 500g of plant material was set on a heating mantle, and the flask was attached to the Clevenger-Arm apparatus after two liters of water were added. The final yield of the oil weighed was 4 ml and the formulation was done with some additives containing 2% of the oil of cymbopogon nardus and 98% of petrolatum stored in refrigerator at 2-8ºC until it is used for the experiment. For acute dermal toxicity test five female rats of age 8-12 week were used , For subacute dermal test 10 animals (5 female and 5 male) with healthy skin were used at each dose level. The females were nulliparous and non-pregnant. Three dose were used, with a control and, petrolatum was used as a vehicle Result, The dermal mean lethal dose LD50 of the C.nardus formulation was found to be above 2000mg/kg but, no mortality sign was observed during the 14 days of study. 28 days dermal application of C.nardus formulation did not produce any toxicity on the behavioral, physical, biological, hematological analytes and gross pathology of the rats at treated dose compared to the control groups. X Conclusion, C. nardus formulation has no dermal toxicity and therefore presents a low toxicological risk. This study concludes that topical application of Rats do not experience systemic toxic responses or acute or subacute negative skin effects from the C. nardus formulation. Key word, Cymbopogon nardus , acute, subacute dermal, sub chronic , toxicity.en-USThesis