Tamrat AbebeZelalem Desalegn2025-08-122025-08-122024-06-13https://etd.aau.edu.et/handle/123456789/6471Female breast cancer incidence has risen to unprecedented levels. It is a complex disease presented with distinct morphology, biological behaviors, clinical outcome and prognosis. Consequently, classification of breast cancer, understanding the molecular events including PIK3CA mutations and exploration of environmental factors including microbiota have a central role in understanding tumor biology. However, there is limited evidences in sub-Saharan Africa (SSA) context including Ethiopia; therefore, this study was conducted to generate evidence-based data to improving breast cancer care in Ethiopia. Additionally, considering the limited infrastructure for breast cancer diagnosis, we did method evaluation between PCR methods and immunohistochemistry to suggest alternative methodsen-USBreast cancerEthiopiaIHCIntrinsic subtypesMicrobiotaMutationOverall survivalPCRPIK3CAPrognostic featuresThesis