Prof. Mahendra PalSelamawit, Mulu2020-06-182023-11-082020-06-182023-11-082014-06http://etd.aau.edu.et/handle/123456789/21769Listeriosis is one of the important food-borne bacterial zoonotic diseases caused by Listeria monocytogenes, as a result of food and environmental contamination as well as zoonotic infections. This disease is becoming an emerging bacterial disease, with low incidence but high case fatality rate. The present study was undertaken to determine the presence of Listeriamonocytogenes in raw meat of market and abattoir. A cross-sectional study was conducted from October 2013 to April 2014 to isolate Listeria monocytogenes from swab samples on sheep meat from abattoir, butcher shops, equipments, and to determine antibiotic resistance profiles of the isolates. A total of 873 swab samples comprising of 384 from the abattoir, 384 from butcher shops were collected using systematic random sampling technique and 105swabs were collected from equipments. Questionnaire survey was conducted to assess the hygienic practices of meat production in raw meat of market and abattoir and possible risk factors regarding the contamination of meat. Listeria monocytogenes was isolated and identified using standard bacteriological techniques. Antimicrobial susceptibility test was also conducted on 36 isolates Listeria monocytogenes. The overall prevalence of Listeria monocytogenes identified was 4.1%. And 2.1%, 5.5% and 6.7% from abattoir, butcher shops and equipments respectively. The study also revealed multi-drug resistant isolates in 24/36 (66.7%) for two or more antimicrobials. In addition, the presence of Listeria monocytogenes attributed to unclean working environment and improper handling of meat till it reaches to the consumer. Preventive measures to avoid the presence of pathogenic Listeria monocytogenes in raw meat and meat products should be undertaken, emphasizing the need for improved hygienic practices during meat production and also during distribution and consumption of the final products.enAddis AbabaAntimicrobial susceptibilityListeria monocytogenesPrevalence and Sheep meatThesis