Dr. Kebede Amenu, Dr. Balako GumiBoki, Negesa2020-11-252023-11-082020-11-252023-11-082020-06http://etd.aau.edu.et/handle/123456789/23552A cross sectional study was conducted in eight towns in Oromia regional state, Ethiopia, from December 2019 to May 2020. The Histoplasma capsulatum var farciminosum and other co-infecting bacteria from characteristic lesions of Epizootic Lymphangitis were iaolated and an effect of Dovyalis abyssinicia on isolated mycelia in-vitro was evaluated. In this study 85 equines suspected with Epizootic Lymphangitis were investigated with clinical observation. In laboratory, the result of Histoplasma capsulatum var farciminosum was isolated (56.47%) from unrupture nodules of pus. The pus sample was also examined under giemsa staining to identify Histoplasma capsulatum var farciminosum. The minimum inhibition concentration of the Dovyalis abyssinicia plant was determined by agar dilution assay. The results showed that Dovyalis abyssinicia had inhibitory effect at ranges of 0.15625mg/ml minimum inhibitor concentration on mycelia. A culture media with no antifungal agent and media containing ketoconazole served as negative and positive control, respectively. Various bacteriological media (Nutrient Agar, Blood Agar, Mannitol Agar, Mackonkey Agar, Edward Agar and Eosin Methylene Blue Agar) were used to isolate contaminants bacteria associated epizootic lymphangitis lesions at Aklilu Lemma Institute of Pathobiology, Addis Ababa University. In concerning of isolation of co-infecting bacteria from three site sample (Holeta, Sebeta and Bishoftu), Staphylococcus species (82.14%), Corynebacterium species (57.14%), Micrococcus species (50.00%), Aeromonus species (50.00%), Klebsiella species (46.42%), Streptococcus species (42.86%), Proteus species (35.71%) and E. coli species (35.71%) were identified. The current study revealed the presence of various contaminant bacterial species which may aggravate an EL and compromise the progress of treatment. The methanol extract of Dovyalis abyssinicia showed strong in vitro growth inhibitory activity against mycelia.enCo-infecting bacteriaEL, HCFDovyalis abyssiniciaThesis