Mamo, Bedaso(DrAbdissa, Rosa2022-06-082023-11-302022-06-082023-11-302014-06http://etd.aau.edu.et/handle/123456789/31949The study was carried out from November 20 13 to April 2014 with the objecti ves of determining the prevalence and source of contamination of E. coli 0157: H7 from municipal abattoir and butcher houses, to assess the public health importance of this organism and to lest the drug sensitivity pattern of the isolates in Oebre-Berhan. A total of 1,132 samples from abattoir and 40 samples from butcher hOllses were examined. Samples from abattoir included hide swabs, internal and external carcass swabs. fecal sample, intestinal mucosal swabs and environmental samples (carcass in contacts, i.c. knife, workers hand, and clothing). Likewise, the samples from butcher houses were swab from carcass, butcher man's hand, cutting board and knife. In addition, stool samples from suspect individuals visiting Debre-Berhan referral hospital were collected. The samples were transportcd in Buffered peptone waler to laboratory of the department of Biological science, Oebre Berhan University in ice box and scrutinized according to international standards. From 220 animals slaughtered, a total of 1100 sampl es were taken and only 6 (0.54%) were found to be positive of £. coli 0157:117. E. coli 0157:H7 was isolated from 2 (0.91 %) of hide swab samples, 3 (1.36%) of feces samples and I (0.45%) of intestinal mucosal swab. There were no isolate found from 32 environmental sampl es, 20 stool samples and 40 swab samples from butcher shops. Prevalence of E. coli 0157:H7 was significantly associated with the cleanness of the hide (P=0.036). All E. coli 0157:H7 isolates were then checked for their susceptibility pattern against ten selected antibiotics. The isolates were completely suscepti ble to 7 antibiotics tested, namely Tetracycline (TE, 30 Ilg), Chloramphenicol (C, 30Ilg), Sulfamethoxazole-Trimitoprim (SXT, 251lg), Ciprofloxacine (eIP, 5Ilg), Nalidixic acid (NA, 30llg), Kenamycine (K . 30llg) and Sterptomycine (S, JOllg) but they were resistant to Amoxicillin (AML, 25 Ilg). Though, the prevalence of E. coli 0157:1-17 in present study is low, the impact of the pathogen should not be underestimated.enAntibiotic, Buffered peptone. Debre Berhan, E. coli 0157: H7, Swab SamplesThesis