Kindie, Samuel (MSc, PhD Candidate)Tsegaye,Aster(MSc, PhD)Aboneh,Fikirte2025-08-122025-08-122022-06https://etd.aau.edu.et/handle/123456789/6459Background: Reference Interval is value obtained by measurement of a particular type of quantity on an individual belonging to a reference group. It is very important in clinical management of patients, maternity follow-up, and monitoring of clinical trials before onset of serious adverse events. In Ethiopia, similar to most developing country laboratory test results are interpreted by using reference intervals which developed for western population .Different factors make the application of western RI during the interpretation of laboratory result leads to unnecessary exclusion of eligible participants, contribute to over reporting of adverse events and increase the number of referrals for clinical investigations. Despite its significance, there have few efforts to establish these tools. Thus, the current study aimed to address the gaps in serum lipid profile and selected electrolytes reference intervals by determining variability with gender among adults and pregnant women in Addis Ababa. Objective: To establish lipid profile (TC, TG, HDL-C, LDL-C) and selected electrolytes (Na, K, Cl) reference interval for apparently healthy adults and pregnant women in Addis Ababa, Ethiopia. Methods: The present community cross-sectional study was conducted from March 1 to June 30, 2020. A total of 522 individuals with age of ≥18 years and pregnant women ≥15 years that lived in the city of Addis Ababa were recruited. After demographic information and a brief medical history of the adults were collected by using questionnaires, important physical examination, and anthropometric measurement were done. Serum lipid profile and selected electrolytes values were determined using Cobas c501 chemistry analyzer. SPSS version 23 and Med Calc ver 20.008 software were used for reference limits determination for adults and pregnant women. Results: Established reference interval for adult female and male adult were; TC [ (120.4-272) and (100.1-243) mg/dl], TG [(51-164) and (46.5-170)mg/dl ]; HDL-c [(25-57) and (16- 52.7)mg/dl], LDL-[(51-170) and (35-162)mg/dl]; Na [(138-149)and (138-150)mmo/l]; K[(3.9- 5.6)and(3.9-5.5 ) mmol/L] and Cl [(101-112)and (99.14-108) mmol/l ]. For pregnant women established RI of lipids and major electrolyte were TC(118-275) mg/dl, TG (64 -304) mg/dl, HDL ( 22-62.5 )mg/dl, LDL(47.8-167)mg/dl, Na(136-143) mmo/l, K(3.65-5.1 ) mmol/L,Cl (100.5-108) mmol/l. Also statistically significant difference was seen for TC, TG, HDL, LDL and Cl between male and female and for TC, TG, Na and K between adult females and pregnant women. Conclusion: The current study established RI to be used for adults and pregnant women of Addis Ababa. There was a difference between the present established reference interval and other reported studies. Thus high or low value seen for lipids (TC,TG and LDL) and electrolyte. Also the value of HDL-c was very low. Significant differences were also seen between genders for most of the analyte, which alerts for further large scale studies, and need careful verification of test kit manufactures’ given values before directly used for the desired purposeen-USAdultReference IntervalsLipid profileElectrolyteFemaleMalePregnant.Thesis