Abegaz Berhanu (PhD)Gebreyohanne Afeworki (PhD)Meless Hailu2018-07-192023-11-082018-07-192023-11-081993-05http://etd.aau.edu.et/handle/123456789/9463In this study a total of 754 individuals were involved. The study population was divided into 3 ~roups: a/200 (27%) pre~nant women, b/l05 (14%) students, and c/449 (59%) patients attending clinics for STDs. Blood samples collected from all members of the study population, were examined for syphilitic infection. A seroprevalence of 13%, 11.2% and 21.6% were found among pregnant "homen, students and STD patients by both RPR and TPHA respectively. The validity of RPR as a screening test was determined using sensitivity, specificity, percentage of positive predictive value and efficiency. Strong positives and positives by the RPR test can be considered as positive for syphilis. Only weak positives require retesting. Endocervical and urethral (male) discharge samples, collected from 233 STD patients in addition to the blood samples, were examined for the presence of Neisseria I{onorrhoeae. The organism was isolated from 68 (29.2%) sub.i ects. Among the isolates, PPNC strains comprised 48 (70%) of the samples. PPNC strains Were resistant to penicillin (100%), ampicillin (96%). bactrim (91%) and kanamycin (22%). Of the nonPPNG strains 25% were resistant to pencillin. 20% to ampicillin, 40% to bactrim and 15% to kanmycin. Both groups were, however. sensitive to erythromycin, tetracycline, chloramphenicol and ceftriaxone.enBiologyThesis