Geyid, Abera (PhD)Kassahun, Jiksa2018-07-032023-11-082018-07-032023-11-082003-06http://etd.aau.edu.et/handle/123456789/5650Sero-epidemiological study of brucellosis was conducted on occupationally exposed humans and dairy cattle in Addis Ababa. A total of 336 human individuals were screened by Rose Bengal Plate Test. Those found to be positive were further subjected to 2-MET. Similarly, 552 sexually matured dairy cattle were screened for RBPT and positives were further subjected to Serum Agglutination Test and Complement Fixation Test. A simultaneous survey was conducted in the farms and Addis Ababa abattoir enterprise to investigate epidemiological factors. An over all seroprevalence of (16/336) 4.8% in humans and 10% (57/552) in dairy cattle was found, taking RBPT+ / 2MET+ in humans and RBPT+ / CFT+ in dairy cattle as a gold standard. A sensitivity and specificity of 88% and 94% in humans and 100% and 68.5% in dairy cattle was observed for RBPT. Where as SAT was 63.3% sensitive and 89.2% specific in dairy cattle. The risk factors contributing to the occurrence of the disease in humans include occupation, sex, raw milk and meat consumption, failure to use detergents after work, and unsafe handling of infected materials. However, herd size and lack of knowledge about brucellosis are the major factors in dairy cattle seropositive animals. Key words: Sero-epidemiological, sensitivity, specificity and risk factorsenSero-epidemiologicalsensitivityspecificity and risk factorsThesis