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Isolation, Identification and Drug Resistance Patterns of Methicilin Resistan Tstaphylococcus Aureus from Mastitic Cow’s Milk from Selected Dairy Farms In and Around Kombolcha

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dc.contributor.advisor Tassew, Asmelash (PhD)
dc.contributor.author Negash, Mesfin
dc.date.accessioned 2018-06-29T07:57:52Z
dc.date.available 2018-06-29T07:57:52Z
dc.date.issued 2015-06
dc.identifier.uri http://localhost:80/xmlui/handle/123456789/4934
dc.description.abstract A cross sectional study was conducted from January 2015 to May 2015 in and around kombolcha town, Eastern Amhara, to isolate and identify methicilin resistant S. aureus and their resistance to different antimicrobials and also identify risk factors associated with the occurrence of dairy cow mastitis. A total of 150 dairy cows were included during the study period. A total of 600 quarters were examined to detect clinical and subclinical mastitis by physical examinations of udder and milk and California mastitis Test, respectively. The prevalence of mastitis was 56%. Out of this, 10% and 46% were clinical and subclinical respectively. Among potential risk factors considered from the farm attributes, age, milking hygiene, parity, and floor system had significant (p=<0.05) effect on the prevalence of mastitis. However, breed, previous treatment and lactation period have not been a significant potential risk factor on the occurrence of mastitis. Staphylococcus aureus was isolated at a rate of 11 (73.3%) and 29 (42%) in clinical and subclinical mastitis, respectively. The result showed that the occurrence of Staphylococcus aureus in clinical mastitis were found to be significantly higher than subclinical mastitis (p=0.028). The present study showed that S. aureus was resistant to penicillin G (100%), Amoxicillin (100%), cefoxtin (42.7%) and tetracycline (77.4%) however all the isolates were found to be totally (100%) susceptible to the gentamycin disc. Out of the resistance S. aureus isolates, 24 (45.3%) were found to be multidrug resistant against 4 antibiotic discs primarily to penicillin G, ampicillin, cefoxtin, and erythromycin. The development of antimicrobial resistance is nearly always as a result of repeated therapeutic and/or indiscriminate use of them. Regular antimicrobial sensitivity testing helps to select effective antibiotics and to reduce the problems of drug resistance development towards commonly used antibiotics. In conclusion, among different risk factors, age of the animal, milking hygiene, parity and floor type were critical for the occurrence of mastitis. Mastitis caused by S. aureus is one of the major problems of dairy cows in milk production in the study area. Key words: antimicrobials, mastitic cows, MRSA, prevalence resistance, risk factors en_US
dc.language.iso en en_US
dc.publisher Addis Ababauniversity en_US
dc.subject antimicrobials en_US
dc.subject mastitic cows en_US
dc.subject MRSA en_US
dc.subject prevalence resistance en_US
dc.subject risk factors en_US
dc.title Isolation, Identification and Drug Resistance Patterns of Methicilin Resistan Tstaphylococcus Aureus from Mastitic Cow’s Milk from Selected Dairy Farms In and Around Kombolcha en_US
dc.type Thesis en_US


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