Background: Management of airway and breathing is one of the most important initial interventions to save the lives of patients and is a cornerstone of excellent emergency care. The first few minutes after an emergency condition are vital to providing these lifesaving interventions. Objective: This study aimed to assess the knowledge, and practice of nurses regarding airway and breathing management who were working in selected hospitals under Addis Ababa health bureau, Addis Ababa, Ethiopia from March to April 30, 2021. Methods: The study used an institutional-based cross-sectional descriptive study with a complete enumeration of all respondents by using the census method during the study period from March to April 30, 2021. A self-administered and structured questionnaire was used for collecting data from the respondents. Data quality was controlled by pre-testing the tools and providing training to the data collectors. The SPSS version 26 software was used for analyzing the data. Mean, frequency, binary, and multiple logistic regression analyses were used. Only Pvalue less than 0.05 was considered statistically significant. Result: About 102 respondents were included in this study with a response rate of 98%. Among those, 54(52.9%) were females. Findings from this study showed that 71.57% and 64.71% of the respondents had good knowledge and practice respectively. Being trained in training related to airway and breathing management before was statistically associated with knowledge and practice on both binary and multiple logistic regression at p<0.05. Conclusion and recommendation: This study showed relatively good knowledge and practice of nurses on airway and breathing management. Even though the finding is good, a significant number of participants had poor knowledge and practice. Therefore, it is very crucial to provide training since it was significantly associated with good practice and knowledge.