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Development of Microfluidic Paper-Based Analytical Device (Μpad) for the Determination of Nitrite and Nitrate Ion in Water Samples

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dc.contributor.advisor Hussen, Ahmed (PhD)
dc.contributor.author Tesfaye, Tilaye
dc.date.accessioned 2021-08-03T06:34:37Z
dc.date.available 2021-08-03T06:34:37Z
dc.date.issued 2020-07-10
dc.identifier.uri http://etd.aau.edu.et/handle/123456789/27546
dc.description.abstract High concentration of nitrate ion in water bodies are considered as public health concern globally, which may cause blue baby syndromes, diabetes, stomach cancer because it produces N-nitroso compounds which are potent carcinogens. Most of the detection techniques are expensive, require trained person and large sample volume. The aim of this study was to develop microfluidic paper-based analytical devices (μPADs) using wax screen-printing technique for the determination of nitrite and nitrate ion in water samples, which is low cost, simple and fast. Nitrite is determined directly by the Griess reaction while nitrate is first reduced to nitrite by zinc powder. μPADs parameters such as sample volume, Griess reagent volume, color development time and Zinc powder loading were optimized and optimal values were found to be 5 μL, 1 μL, 10 minutes and 25 mg, respectively. Under optimal condition, the limits of detection for nitrite and nitrate ion were found to be 0.16 and 0.87 ppm, respectively. This level of sensitivity observed in μPAD is adequate to determine the threshold concentration of nitrite (1 ppm) and nitrate (50 ppm) in drinking water set by WHO. Recovery test using the fabricated μPAD on spiked tap water revealed more than 95% recovery for both target analytes. It was comparable with the recovery obtained by using the standard method UV-vis (> 96%) which indicates the validity of the developed method. Furthermore, the application of μPADs for the analysis of nitrite and nitrate in Dire Dawa ground water samples showed below detection limit and 70.5 ppm, respectively. The concentration measured using μPADs were in an excellent agreement with the values obtained from UV-Visible spectrophotometry. This implies potential use the μPADs for environmental monitoring of nitrate and nitrite in resources limited areas without need for expensive benchtop analytical devices. en_US
dc.language.iso en en_US
dc.publisher Addis Ababa University en_US
dc.subject Μpads en_US
dc.subject Nitrate en_US
dc.subject Nitrite en_US
dc.subject Griess Reagent en_US
dc.subject Wax Screen-Printing en_US
dc.subject Colorimetric Detection en_US
dc.title Development of Microfluidic Paper-Based Analytical Device (Μpad) for the Determination of Nitrite and Nitrate Ion in Water Samples en_US
dc.type Thesis en_US

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