Background: Cervical cancer is preventable and remains a leading cause of avoidable death among women in the world. In developing country, the knowledge of screening for cervical cancer behavior still very low, the converge of cervical cancer screening was from 2.0% to 20.2% in urban areas and from 0.4% to 14.0% in rural areas. Previous findings of research were indicated that the magnitude of screening for cervical cancer in Ethiopia is very low, which is about 0.6%, this indicated the knowledge of women is low because, of the women did not come to screened for cervical cancer. Materials and Methods: A cross-sectional study design was used. Data were collected using a structured self-administered questionnaire. Analysis was conducted using SPSS version 23. Results: About 312 urban health extension workers were included in this study. The mean age (±) standard deviation) of the study participant was 20.41 years ± SD, 3.73 years). Based on this finding, about 48.4% of the participants have good level of knowledge about cervical cancer screening and about 43.3% of the participant who were source of information were health professionals. A multiple logistic regression were performed to identify the existence and significance of association, work experience and monthly income were significantly associated [AOR = 4.32, 95% CI: (1.71, 10.94], monthly income [AOR = 3.75, 95% CI: (1.49, 9.41) and (A0R= 3.08 95% CI: (1.06, 8.98) were associated. Conclusion: This study has revealed that the knowledge towards cervical cancer screening was poor among urban health extension workers of Addis Ababa, health centers. Generally, information could be subordinate than seems in this explore. In conclusion, knowledge towards cervical cancer screening and when should be screening showed several misunderstandings. Based on this misunderstanding, continuing delivering information to encourage screening including health extension workers to raise the knowledge of women.