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Determination of Selected Metals in The Stems of Salix Subserrata („Akya‟), Sida Cuneifolia („Chifrig‟) and Clausena Anisata („Limich‟) Collected From Three Different Areas of Ethiopia

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dc.contributor.author Entele, Wubalem
dc.date.accessioned 2020-09-16T08:18:29Z
dc.date.available 2020-09-16T08:18:29Z
dc.date.issued 2020-02-02
dc.identifier.uri http://etd.aau.edu.et/handle/123456789/22335
dc.description.abstract Pencil sized sticks are fashioned from certain plant parts and are chewed on one end until they become frayed into a brush and the brush end is used to clean the teeth in a similar manner to the toothbrush. The plant parts when used in this manner are commonly referred as the “chewing stick”. Their use still continues in the modern era of dentistry. It is inexpensive and easily available in rural areas. Further they are used for customary and religious reasons as well. In this study the levels of essential and non-essential metals in the three plants (Salix subserrata („Akya‟), Sida cuneifolia („Chifrig‟) and Clausena anisata („Limich‟)) were determined in samples collected from three selected areas (Muger, Sendafa and Holleta) of Ethiopia. After proper sample pretreatment, for Clausena anisata („Limich‟) 3 mL of HNO3 (69.5%) and 1 mL of HClO4 (70%) for 2:15 h at a temperature of 240 oC, for Sida cuneifolia („Chifrig‟) 4 mL of HNO3 (69.5%) and 1 mL of HClO4 (70%) for 3 h at a temperature of 210 oC and for Salix subserreta („Akeya‟) 3 mL of HNO3 (69.5%) and 1 mL of HClO4 (70%) for 2 h at a temperature of 270 oC were the optimized digestion conditions. Then using the optimized conditions sample preparation was made and levels of metals were determined by microwave plasma-atomic absorption spectrometry (MP-AES). The mean concentration levels of the metals in the three plant samples collected from the three sampling areas indicated that higher concentrations of Ca and Fe are present in all the samples collected from the three areas. The overall mean concentrations of the metals (mg/kg) in the Salix subserrata, Sida cunnefolia, and Clausena anisata samples collected from the three sampling areas can be ordered as Ca (14150-25914) > Fe (514-1191) > Al (103-1263) > Zn (152-196) > Mg (46-102) > Ni (4-160) > Mn (25-78) > Cu (13-20) > Cr (7-8). The accuracy of the optimized procedure was evaluated by analyzing the digest of the spiked samples with standard solution and the percentage recoveries varied from 92% to 104%, which is good and is in the allowed range of 90 ± 10%. ANOVA indicated that there is no significance difference between the mean concentrations of Ca among the Salix subserrata samples, of Mg, Cu, Zn and Ca among the Sida cunnefolia samples and of Al, Cu, Cr, and Ni among the Clausena anisata samples, but there is a significant difference for the other studied metals among the corresponding plant samples at 95% confidence level. Since the toxic elements Cd and Pb in the plant samples are not detected, it is possible to conclude that a person who consumes plants from these sampling areas is free from the risks of Cd and Pb toxicity. en_US
dc.language.iso en en_US
dc.publisher Addis Ababa University en_US
dc.subject Determination of Selected Metals en_US
dc.subject Stems of Salix Subserrata en_US
dc.subject Sida Cuneifolia en_US
dc.subject Akya en_US
dc.subject Chifrig en_US
dc.subject Clausena Anisata en_US
dc.subject Limich en_US
dc.subject Collected from Three Different Areas en_US
dc.subject Ethiopia en_US
dc.title Determination of Selected Metals in The Stems of Salix Subserrata („Akya‟), Sida Cuneifolia („Chifrig‟) and Clausena Anisata („Limich‟) Collected From Three Different Areas of Ethiopia en_US
dc.type Thesis en_US


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