Background: Annually, throughout the world, an estimated 271 million people suffer from work related injuries, and 2 million die from the injury. In recent years, in developed countries the occupational health and safety of workers has improved, but it is not true in developing countries. Ethiopia is categorized among under developed countries and do not have much data on health and safety. Objective: The objective of the study was to assess the knowledge and practice of factory workers on occupational safety information. Methods: A descriptive cross- sectional study was conducted in Kality Metal Product and Ethiopian Iron and Steel factories in March 2007. A total of 417 study subjects were randomly selected from the two factories according to their worker number proportions. The data was collected using the questionnaires, focus group discussion, and observational checklists. The questionnaire was pre-tested for their accuracy and validation prior to the actual study and the necessary adjustments were done accordingly. The data were entered after being encoded and analyzed using Epi info and SPSS. Results: The mean (standard deviation) age of the respondents was 39.56± (11.08). Sixty nine percent of the respondents recognized at least one safety sign. Two hundred eighteen (52.3%) respondents got training on different occupational health and safety information and 71.5% of the respondents used glove and 48.2% used boots. Safety recommendation, sex, and work experience were found to be the determinant factors of respondents knowledge level [OR: 2.394(1.439, 3.984)], [OR: 2.398(1.169, 4.922)], and [OR: 1.934(1.178, 3.174)] respectively. Health and safety training was found to be a positive determinant factor for PPD usage [OR: 1.970(1.146, 3.386)]. Conclusion and recommendation: The workers have less knowledge level compared to other studies. Health and safety recommendations have positive effects on both knowledge levels and safe practices. Health and safety training has positive effects on safe practice but did not impact knowledge. Health and safety recommendation should be given to the workers. Safety signs should posted at a reasonable work sites. Keywords: occupational health and safety information,knowledge,practice