Background: Poisoning exposures continue to be a significant cause of morbidity and mortality worldwide. The lack of facilities, treatment resources, and antidotes in hospitals may affect the treatments provided and outcomes. Objective: Assessment of availability of treatment resource in emergency department for the management of acute toxic exposures and poisoning detection in selected governmental hospitals, Addis Ababa, Ethiopia. Methodology: Hospital based descriptive cross sectional study was applied to assess availability of treatment resource for the management of acute toxic exposures and poisoning in selected governmental hospitals from December 2016 to June 2017. Data were collected using semi-structured questionnaire and the data obtained was analysed using SPSS and the result was presented using tables Chart and graph. Result: Over the study period, 920 poisoning cases were listed in the selected hospital registries. Among them, Organo phosphate poisoning was the most top leading cause of acute poison (18.8%, 173/920), Followed by Bleach (Sodium hypochlorite) poison (18.6%, 172/920). In all the hospitals all form of Charcoal and Sodium Sulphate were not available. Four of the five hospitals have had Orogastric tube and magnesium Sulphate. Only Nasogastric tube out of the ten decontamination resource had available in all hospitals. None of the studied hospitals have the stock of all either WHO or Ethiopian essential drug list recommended antidotes and none had fomepizole, pralidoxime, charcoal, sorbitol, ipecac, polyethylene glycol, deferoxamine dimercaprol, glucagon, and Protamine sulphate. In surveyed hospitals peritoneal dialysis was not available. Conclusion: For the majority of the surveyed hospitals the resources which are essential for treatment and management of poisoned patient are not sufficient. Furthermore, the available resources and the pattern of causes of poisoning show discrepancy. Recommendation: Based on the pattern of causes of poisoning it is better if the selected anti- dotes, stabilization, decontamination and elimination resource are available. Keywords Decontamination _ Elimination enhancement _ Stabilization resources _Availability_ Hospital _ Ethiopia _Poisoning.