A new tripodal ligand 2, 4, 6-Tris (N-Salicylidenehydrazino)-s-Triazine (L) was synthesized by the condensation of salicylaldehyde with 2, 4, 6-tris-(hydrazine)- s-triazine(THSTZ) in acetonitrile. Copper complex of the ligand was synthesized in chloroform-methanol 50% v/v medium. The ligand and the metal complex were characterized by employing spectral (IR,Uv-Vis,AAS, NMR), conductance and magnetic susceptibility studies. The purities of the compounds were established by TLC. The studies support formation of stable metal complex corresponding to the formulae: [Cu(II)LH2O] .4H2O. Conductivity measurement indicates that the complex is a non-electrolyte. Spectral and Magnetic data suggest dibasic ONN donor behavior of the ligand towards Cu(II). Square planar geometry for Cu2+ complex has been proposed. The ligand, metal complex and related compounds were screened for antimicrobial activities against Escherichia coli, Pseudomonas eruginosa, Staphylococcus aureus and Bacillus cereus bacteria by employing tetracycline as test control agent. The result showed that both the ligand and the metal complexes were inactive, while the ligand precursor (THSTZ) and the metal salt (CuCl2.2H2O) were active. As such derivatization and the complexation can help in detoxication purposes. Key words: 2, 4, 6-Tris (N-Salicylidenehydrazino)-s-Triazine (L), tripodal ONN donor, Cu2+ complex, Square planar geometry, antimicrobial.