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Title: Verification and comparison of Sysmex KX-21N, Cell-Dyn 1800 And Horiba ABX Micros ES 60 automated hematology analyzers at Tikur Anbessa Specialized Hospital Addis Ababa, Ethiopia
???metadata.dc.contributor.*???: Aster Tsegaye (MSc, PhD)
Jemal Alemu (MSc, PhD candidate)
Mikias Negash (MSc)
Henok Kebede
Keywords: Method comparison, performance evaluation, verification of hematology analyzers Sysmex KX-21, Horiba ABX micros ES 60, Cell-Dyn 1800, hematology
Issue Date: Jun-2017
Publisher: AAU 2017
Abstract: Background: Laboratory testing plays a crucial role in the detection, diagnosis and treatment of disease in patients. Analytical evaluation of a new analyzer or method should be done in order to confirm the declared specifications before its introduction into the routine use. Agreement of all automated hematology analyzers must be carried out in all laboratories. But, the practice of using automated analyzers interchangeably without evaluation is common in our country including Tikur Anbessa specialized hospital (TASH). Objective: To verify performance and compare agreement of Cell-Dyn 1800, Sysmex KX-21N and Horiba ABX micros ES 60 hematology analyzers found in TASH. Methods: A cross sectional study was conducted to verify performance and assess agreement between Cell-Dyn 1800, Sysmex KX-21N and Horiba ABX micros ES 60 hematology analyzers at Tikur Anbessa specialized hospital, Addis Ababa, Ethiopia. The study period was from February to May 2017. According to the CLSI guideline a total of 267 samples were analyzed in duplicates on the three analyzers to verify and compare them. Data cleaning and analysis were performed by Microsoft excel 2007 and SPSS version 20. Result: We found that minimal carryover (< 0.5%) and excellent linearity for white blood cell, hemoglobin, red blood cell and platelet (r > 0.999). Precision was good for all major CBC parameters with % CV< 2.0. Agreement between most of the parameters of the analyzers lies in the limits of agreement for more than 95% of cases according to the Bland-Altman plot analysis. Pearson correlation and linear regression graph showed that the three analyzers were well correlated (r > 0.900) for major CBC parameters. The correlation of WBC differential- neutrophil and lymphocyte were excellent (r > 0.920). Poor correlation was observed in mixed cells (r=0.005) especially in the high levels between Horiba and Sysmex. There was also moderate correlation in mean platelet volume (MPV) between analyzers of Horiba and Sysmex (r= 0.593 and r=0.572) at normal and high levels respectively. Conclusion: The overall performance of the three analyzers is excellent. They agree well with each other and can be used interchangeably with care in mixed cells and MPV.
Description: A Thesis Submitted to the Department of Medical Laboratory Sciences, School of Allied Health Science, College of Health Science, Addis Ababa University, in Partial Fulfillment of Masters of Science Degree In Clinical Laboratory Sciences (Hematology and Immunohematology Track).
Appears in Collections:Thesis - Medical Laboratory

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