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Title: Evaluation of Techniques for the Diagnosis of Strongyloides Stercoralis in HIV Positive and HIV Negative Individuals in Selected Health Institutions in Addis Ababa
???metadata.dc.contributor.*???: Prof. Beyene Petros
Hailegebriel, Tamirat
Issue Date: Jul-2006
Publisher: Addis Ababa University
Abstract: A total of 351 individuals, 226 HIV positive and 125 HIV negative, were examined by nutrient agar plate, Baermann method, direct fecal smear, concentration method, Harada Mori filter paper and charcoal culture in this study. Among these 139(39.6%) were positive for different type of intestinal parasites. The common intestinal parasites detected in this study were S. stercoralis, E. histolytica/dispar, A. lumbricoides, B. hominis, G. lamblia, hookworm, C. parvum, and I. belli. Out of these parasites, more than 90% of S. stercoralis and all cases of C. parvum and I. belli were detected from HIV positive subjects. Although different intestinal parasites were detected from HIV patients, S. stercoralis was the predominant infection detected by nutrient agar plate and was associated with chronic diarrhea in the HIV/ AIDS patients included in the study. Since S. stercoralis is known to exist as an asymptomatic chronic infection, more classical parasitological methods such as direct and concentration methods were not as sensitive as the agar plate culture and the Baermann method. The present study showed that the nutrient agar plate culture method was the most sensitive method for the detection of S, stercoralis infection. It detected 97.7% of the infection among the total study subjects. It furthermore detected 90.7% of S. stercoralis infection in HIV positive patients with and without diarrhea. By using this method, it was possible to show that 76.9% of S. stercoralis infection to be associated with diarrhea in HIV positive patients (P<0.001). The agar plate culture method has shown a strong association between HIV/ AIDS and S. stercoralis infections (P<0.001). Thus, this sensitive and specific diagnostic method can be used to detect early and latent infections of S. stercoralis in HIV/ AIDS patients.
Description: A Thesis Submitted to the School of Graduate Studies Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biology
Appears in Collections:Thesis - Biology

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