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Title: Effect of Extracts from Two Artemisia Species
Other Titles: Artemisia Afra and Artemisia Rehan on Isolated Smooth Muscle Tissues in Vitro
???metadata.dc.contributor.*???: Dr. Yalemtsehay I Mekonnen
Mulatu, Abiyselassie
Issue Date: Jul-2004
Publisher: Addis Ababa University
Abstract: Ethanolic and aqueous extracts of dried leaf and root of Artemisia afra and Artemisia rehan were tested on isolated mouse duodenum, guinea pig ileum and mouse uterine strips in vitro. The potential spasmolytic (relaxant) and spasmogenic (contractile) effect of the plants were studied in these smooth muscle tissue preparations. Different concentrations of each extract of the plants (ranging from 20-200 pg/ml) were tried out in the presence of agonist controls, ACh (in MD and MU) and histamine (in GPI) as contraction stimulators. A positive antagonist control, papaverine was used as a smooth muscle relaxant in GPI. In most of the ethanolic extracts , there were significant dose-dependent reductions of ACh and histamineinduced contractions in both tissues (P < 0.05). Both spontaneous rhythmic and agonistinduced contractions of MD and GPI were greatly reduced by ALE (56% and 35% respectively) and RLE (65% and 56% respectively) at maximal dosages, which suggests spasmolytic property of the crude extracts. All the extracts (200 pg/ml) except ALE and RLE were less potent than papaverine (1.5 pg/ml) in the experiment of GPI. Neither the aqueous nor ethanol extracts of the two plant species caused significant contractile or relaxant effects on MU. All in all the results in the present study pinpoint that the plants may possess spasmolytic as well as spasmogenic properties based upon the appropriate use of extractive solvent. The results also support the traditional folk use of the aerial and root parts of the plants more often for stomach pains and intestinal cramps than for fertility control purposes.
Description: A Thesis Presented to the School of Graduate Studies of the Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biology
Appears in Collections:Thesis - Biology

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