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Title: In Vitro Investigation of Antimicrobial Activities of Albizia Gummifera and Albizia Anthelmintica on Major Bacterial Uropathogens Isolated from Adult Patients in Addis Ababa
???metadata.dc.contributor.*???: Dr. Aberra Geyid
Dr. Asfaw Debella
Kassaye, Semere
Keywords: Albizia Anlhelmintica;Albizia Gummifera;MIC;Uropathogenic E. Coli;UTI
Issue Date: Jul-2006
Publisher: Addis Ababa University
Abstract: A study involving the Collection, Isolation, Identification and Sensitivity screening of major bacterial uropathogens and also the antibacterial activity of two traditionally used plants against the isolates of these uropathogens for various ailments in Ethiopia was conducted at Ethiopian Health and Nutrition Research Institute (EHNRI). The specimens for the isolation of the bacterial uropathogens were collected from adult out-patients with clinically proved symptomatic Urinary tract infections and referred to the EHNRI Reception of the Laboratory Core Service and the Arsho Medical Laboratory by physicians from various Hospitals or Clinics, Out of the total 241 clean catch mid stream urine samples 66 were revealed to have significant bacteriuria. The most frequently isolated species was E.coli (77.3%) followed by Klebsiella spp. (12.1%), Pseudomonas spp. (4.5%), Proteus spp. (4.5%) and Enlerobacter spp. (1.5%). In this study, high level multidrug resistance was observed particularly for commonly prescribed antibiotics: Amoxycillin (69.7%), Ampicillin (64.6%), Tetracycline (53.0%) and Co-trimoxazole (43.9%) using disc diffusion method. The in vitro antibacterial activities of 80% methanol crude extracts and their hydro alcoholic solvent fractionates prepared from both seeds of Albizia gummifera and Albizia anlhelmintica were tested for their antibacterial activity against the clinical isolates of E.coli (31), Klebsiella spp.(8), Pseudomonas spp.( 3 ), Proteus spp.( 3 ) and also standard reference strains of E.coli ATCC,25922 and P.aeruginosa ATCC,27853 using agar dilution method. The chloroform fraction didn’t exhibit any activity while n-butanol fraction was found to have strong antibacterial effects as compared to the aqueous solubilized fraction which showed antibacterial activity only against E.coli and Klebsiella spp. at lOOOpg/ml. Minimum inhibitory concentration values of n-butanol fraction against the clinical isolates of E.coli showed stronger activity than the commonly used broad spectrum antibiotics: Tetracycline and Amoxycillin. Additionally, a better activity was exhibited by this n-butanol fraction against the clinical isolates of Klebsiella spp. and Pseudomonas spp. than Amoxycillin. Over all, in this study it was found that n-butanol fraction of 80% methanol crude plant extracts of both plants may contain bioactive compounds with therapeutic activity.
Description: A Thesis Submitted To Graduate Studies Program Addis Ababa University in Partial Fulfillment of the Requirments for the Degree of Masters of Science in Applied Microbiology.
Appears in Collections:Thesis- Microbial, Cellular and Molecular Biology

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