Skip navigation

Please use this identifier to cite or link to this item:
Title: Identification of Serotypes and Assessment of Multiple Drug Resistance in 360 Shigella Isolates
???metadata.dc.contributor.*???: Dr. Don J. Brenner
Dr. M. Toucas
Gebre-Yohannes, Afeworki
Issue Date: Jun-1980
Publisher: Addis Ababa University
Abstract: Three hundred and sixty urban and rural Shigellae isolates were analysed in respect to serogrouping, serotyping, biochemotyping and drug resistance. S. flexneri (50.55%) was most common, followed by S. dysenteriae (32.78%), S, boydii (11.39%) and S. sonnei (5.28%). Isolation rates of S. dysentoriae, S. flexneri and S. sonnei were comparable in rural and urban areas, except for 5. boydii which is more common in urban areas (significant at P=0.05). Out of the thirty-two known Shigolla serotyPes, twenty-two were identified in this study (i.e. S. dysenteriae 1, 2, 3, 4, 6, 21 A. flexnerk 1, 2, 3, 4, 61 8. boydii I, 2, 3, 4, 5, 8, 9, 10, 12, 14; and S. sonnei). Urban isolates were represented by 22 serotypes compared to only 11 in rural areas. S. dysenteriae serotype 1 (Shiga's bacillus) was more common in rural areas (34.48%) than in urban areas (17.22%), and this differenoo ie significant (Pe0.05). Nineteen patterns of drug resistance were observed, with Tau (21.11%), TCACbSSu (19.72%) and TSSu (12.50%) being comparatively more common. There were nine patterns of drug resistance in serogroup A, 12 patterns in B, 8 patterns in C, and 5 patterns in D. TCACbSSu pattern (53.39%) in serogroup A, Tau (41.76%) in B, and TSSu (26.31%) in D wore observed. Within serogroup A, 81.82% of S. dysenteriae serotype 1 was associated with the TCACbSSu pattern. There were 17. Patterns of resistance in urban areas compared to 10 in rural areas. The prominence of TCACbSSu pattern in rural areas was related to the high isolation rate of S. dysenteriae type 1 in these places. In rural areas: 14.04% of Shigella isolates were classified as sensitive to all drugs tested, compared to 26.37% in urban arena and this difference is significant (Bm0.05). Identification of rare biochemotypes included: a) three strains of mannitol negative 8. flexneri type 6, b) a strain of gas producing 8. boydii type 14, and c) a strain of 'invasive' B. ooli (0:164) serologioally cross reacting in 8. dvaenteriae sorotype 3 antiserum. Based on the present study, it is recommended that: a) a further study of Shigolla be persuud, b) a Shigella reference center be established, 0 an improvement of sanitation be stressed, d) chemotherapy be discouraged and a) a national policy for drug sale be enacted.
Description: A Thesis Submitted to School of Graduate Studies Addis Ababa University in Partial Fulfillment of the Requirements for the Degree Master of Science in Biology.
Appears in Collections:Thesis - Biology

Files in This Item:
File Description SizeFormat 
Afeworki Gebre-Yohannes.pdf3.51 MBAdobe PDFView/Open
Show full item record

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.